|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Pig (Porcine) Alternatives|
|Conjugate||This CD8 antibody is un-conjugated Alternatives|
Cytotoxicity Test (CyTox), Flow Cytometry (FACS), Immunoprecipitation (IP)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN371299) Images
|Specificity||This antibody reacts with Porcine CD8 alpha (Mr. 32-34 kDa). (Ref-1-3)|
|Characteristics||Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN371299) Images back to top
|Alternative Name||CD8 (CD8A Antibody Abstract)|
|Background||The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN371299) Images back to top
Flow Cytometry. (Ref.1-3)Immunoprecipitation Cytotoxicity Assays (Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN371299) Images back to top
|Buffer||100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD8 Antibody (ABIN371299)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Yamada, Sachs, DerSimonian: "Human anti-porcine xenogeneic T cell response. Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 11, pp. 5249-56, 1995
Smith, Sablinski, Arn, Myers, Rosengard, Uckun, Sachs: "In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine." in: Journal of immunotherapy with emphasis on tumor immunology : official journal of the Society for Biological Therapy, Vol. 16, Issue 2, pp. 105-14, 1995
Pescovitz, Lunney, Sachs: "Preparation and characterization of monoclonal antibodies reactive with porcine PBL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 1, pp. 368-75, 1984