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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|Flow Cytometry (FACS)||89 Antibodies|
|Functional Studies (Func)||6 Antibodies|
|Immunoaffinity Chromatography (IAC)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||27 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||2 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD8 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Immunoaffinity Chromatography (IAC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|16 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN967471) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||High-molecular-weight rat thymocyte glycoproteins|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN967471) Images back to top
|Alternative Name||CD8a (CD8A Antibody Abstract)|
|Background||The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32 kDa alpha chain of the CD8 differentiation antigen. A truncated CD8 alpha' isoform has not been detected in the rat. The CD8 alpha and beta chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). Intestinal intrapithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. OX-8 antibody does not react with resting CD4+ T helper cells. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase Ick. Macrophages have also been reported to express CD8 alpha and beta chains, which are involved in signal transduction. Soluble OX-8 mAb partially blocks in vitro MLR and CTL activity. This antibody is routinely tested by flow cytometric analysis.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN967471) Images back to top
|Application Notes||For IHC, we recommend the use of purified OX-8 mAb in our special formulation for immunohistochemistry.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN967471) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4° C.|
References for anti-CD8 Antibody (ABIN967471)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
|Product cited in:||
Scriba, Grau, Steiniger: "Phenotype of rat monocytes during acute kidney allograft rejection: increased expression of NKR-P1 and reduction of CD43." in: Scandinavian journal of immunology, Vol. 47, Issue 4, pp. 332-42, 1998 (PubMed).
Mitnacht, Bischof, Torres-Nagel et al.: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).
Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997 (PubMed).
Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." in: Transplantation, Vol. 60, Issue 6, pp. 594-601, 1995 (PubMed).
Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).
Brideau, Carter, McMaster et al.: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980 (PubMed).
Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." in: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, 1984 (PubMed).
Mason, Arthur, Dallman et al.: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." in: Immunological reviews, Vol. 74, pp. 57-82, 1983 (PubMed).
Johnson, Gagnon, Barclay et al.: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985 (PubMed).
Bierer, Sleckman, Ratnofsky et al.: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).
Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).
Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." in: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, 1992 (PubMed).
Torres-Nagel, Kraus, Brown et al.: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).
Hirji, Lin, Bissonnette et al.: "Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6004-11, 1998 (PubMed).
Kühnlein, Park, Herrmann et al.: "Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 979-86, 1994 (PubMed).
Classon, Brown, Garnett et al.: "The hinge region of the CD8 alpha chain: structure, antigenicity, and utility in expression of immunoglobulin superfamily domains." in: International immunology, Vol. 4, Issue 2, pp. 215-25, 1992 (PubMed).
ImagesProduct Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN967471) back to top