You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Rat (Rattus)
1071
538
167
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1304
519
205
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Biotin
263
219
143
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
1640
470
373
351
289
285
264
246
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967472
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30849865 ABIN967471 BR IAC IHC (fro) FACS IHC (zinc) IHC (p) IP WB Mouse IgG1, kappa Log in to see OX-8 16
0.30849865 ABIN2661845 FACS FITC Mouse IgG1 kappa Log in to see OX-8 8
0.30849865 ABIN2658736 FACS APC Mouse IgG2a kappa Log in to see G28 8
0.30849865 ABIN2663632 FACS PE Mouse IgG1 kappa Log in to see OX-8 8
0.30849865 ABIN2663636 FACS PE Mouse IgG2a kappa Log in to see G28 8
0.30849865 ABIN2168242 FACS PE Mouse IgG1 Log in to see
0.30849865 ABIN2166826 FACS FITC Mouse IgG1 Log in to see
0.30849865 ABIN4295972 Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7 5
0.30849865 ABIN2166828 FACS FITC Mouse IgG1 Log in to see
0.30849865 ABIN2166827 FACS FITC Mouse IgG1 Log in to see
0.30849865 ABIN2167356 IHC (af) IHC (fp) FACS IP WB Mouse IgG1 Log in to see
0.30849865 ABIN2167355 IHC (af) IHC (fp) FACS IP Mouse IgG1 Log in to see
0.30849865 ABIN2166825 IHC (af) IHC (fp) FACS IP WB Mouse IgG1 Log in to see
0.30849865 ABIN2472531 Func IHC (fro) FACS IP IHC (p) WB Mouse IgG1 Log in to see OX-8 4
0.30849865 ABIN133184 FACS Mouse IgG1 Log in to see OX-8
0.30849865 ABIN2679563 FACS APC Mouse IgG1 kappa Log in to see OX8
0.30849865 ABIN2679562 FACS FITC Mouse IgG1 kappa Log in to see OX8
0.30849865 ABIN2679565 FACS PE-Cy7 Mouse IgG1 kappa Log in to see OX8
0.30849865 ABIN2679567 FACS PerCP-eFluor® 710 Mouse IgG1 kappa Log in to see OX8
0.0010455068 ABIN120396 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see OX-8 8

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
ELISA 3 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Flow Cytometry (FACS) 121 Antibodies
Functional Studies (Func) 7 Antibodies
Immunoaffinity Chromatography (IAC) 2 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Rat (Rattus)
(1071), (538), (167), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1304), (519), (205)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Biotin
(263), (219), (143), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(1640), (470), (373), (351), (289), (285), (264), (246), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 15 references available
Supplier Log in to see

Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN967472) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen High-molecular-weight rat thymocyte glycoproteins
Clone OX-8
Isotype IgG1, kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN967472) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32 kDa alpha chain of the CD8 differentiation antigen. A truncated CD8 alpha' isoform has not been detected in the rat. The CD8 alpha and beta chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). Intestinal intrapithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. OX-8 antibody does not react with resting CD4+ T helper cells. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase Ick. Macrophages have also been reported to express CD8 alpha and beta chains, which are involved in signal transduction. Soluble OX-8 mAb partially blocks in vitro MLR and CTL activity. This antibody is routinely tested by flow cytometric analysis.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN967472) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN967472) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD8 Antibody (ABIN967472)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Hirji, Lin, Bissonnette, Belosevic, Befus: "Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6004-11, 1998

Product cited in:

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Scriba, Grau, Steiniger: "Phenotype of rat monocytes during acute kidney allograft rejection: increased expression of NKR-P1 and reduction of CD43." in: Scandinavian journal of immunology, Vol. 47, Issue 4, pp. 332-42, 1998

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." in: Transplantation, Vol. 60, Issue 6, pp. 594-601, 1995

Kühnlein, Park, Herrmann, Elbe, Hünig et al.: "Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 979-86, 1994

Classon, Brown, Garnett, Somoza, Barclay, Willis, Williams: "The hinge region of the CD8 alpha chain: structure, antigenicity, and utility in expression of immunoglobulin superfamily domains." in: International immunology, Vol. 4, Issue 2, pp. 215-25, 1992

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." in: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, 1984

Mason, Arthur, Dallman, Green, Spickett, Thomas: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." in: Immunological reviews, Vol. 74, pp. 57-82, 1983

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN967472) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (Biotin) (ABIN967472) The expression of CD8a on rat splenocytes. Single-cell suspensions of Lewis splenocyt...
 image for anti-CD8 antibody (CD8a Molecule)  (Biotin) (ABIN967472) Stained with Avidin-FITC