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anti-Rat (Rattus) CD8 antibody for Immunofluorescence

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Rat (Rattus)
1059
532
166
68
47
40
25
22
20
20
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
259
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1617
451
356
342
284
281
254
238
214
37
33
25
23
17
14
10
7
6
6
6
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120395
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843406 ABIN255127 ICC FACS IF FITC Mouse IgG1 Log in to see OX-8
0.0010843406 ABIN1584215 IF FITC Rabbit N-Term Log in to see Polyclonal
0.0010843406 ABIN4295971 Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4295972 Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296117 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296118 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 405 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296119 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 647 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296120 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 700 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296121 FACS ICC IF IHC (fro) IHC (p) DyLight 650 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296122 FACS ICC IF IHC (fro) IHC (p) DyLight 680 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296123 FACS ICC IF IHC (fro) IHC (p) DyLight 488 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296124 FACS ICC IF IHC (fro) IHC (p) DyLight 755 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296125 FACS ICC IF IHC (fro) IHC (p) DyLight 550 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296126 FACS ICC IF IHC (fro) IHC (p) DyLight 350 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296127 FACS ICC IF IHC (fro) IHC (p) DyLight 405 Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296128 FACS ICC IF IHC (fro) IHC (p) DyLight 405LS Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN4296129 ICC IF IHC (fro) IHC (p) Biotin Rat IgG2a kappa Log in to see 53-6-7
0.0010843406 ABIN1854069 ICC FACS IF FITC Mouse IgG1 Log in to see
0.0010843406 ABIN1854070 ICC FACS IF Biotin Mouse IgG1 Log in to see
0.0010843406 ABIN1854071 ICC FACS IF PE Mouse IgG1 Log in to see

Top referenced anti-CD8 antibody for Immunofluorescence

Similar anti-CD8 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
ELISA 3 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Functional Studies (Func) 7 Antibodies
Immunoaffinity Chromatography (IAC) 2 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Rat (Rattus)
(1059), (532), (166), (68), (47), (40), (25), (22), (20), (20), (19), (16), (15), (13), (12), (12), (11), (11), (10), (10), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1285), (512), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(259), (217), (142), (86), (52), (42), (40), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1617), (451), (356), (342), (284), (281), (254), (238), (214), (37), (33), (25), (23), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN120395) Images
Specificity This antibody recognises the CD8 alpha cell surface antigen. Clone MRC OX-8 is suitable for use in in vitro blocking studies. We recommend the use of SM259A for this purpose. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde)
Purification Affinity chromatography on Protein G
Immunogen Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Clone OX-8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN120395) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 is expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 24930
NCBI Accession NP_113726
UniProt P07725
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN120395) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN120395) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN120395)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Popov, Dela Cruz, Barber, Chiu, Inman: "The effect of an anti-HLA-B27 immune response on CTL recognition of Chlamydia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3375-82, 2001

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Farwell, Roberts, Rabinowitz: "The effect of ribosomal protein S1 from Escherichia coli and Micrococcus luteus on protein synthesis in vitro by E. coli and Bacillus subtilis." in: Molecular microbiology, Vol. 6, Issue 22, pp. 3375-83, 1993

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985

Bukovský, Presl, Zidovský: "Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy." in: Immunology, Vol. 52, Issue 4, pp. 631-40, 1984

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." in: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, 1984

Cantrell, Robins, Brooks, Baldwin: "Phenotype of rat natural killer cells defined by monoclonal antibodies marking rat lymphocyte subsets." in: Immunology, Vol. 45, Issue 1, pp. 97-103, 1982

Dallman, Mason, Webb: "The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells." in: European journal of immunology, Vol. 12, Issue 6, pp. 511-8, 1982

Lyscom, Brueton: "Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies." in: Immunology, Vol. 45, Issue 4, pp. 775-83, 1982

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980