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anti-Rat (Rattus) CD8 antibody for Immunofluorescence

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine), Rat (Rattus)
567
511
134
95
44
28
19
14
13
13
12
10
7
4
2
2
1
1
Host
Rat
739
482
186
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
158
152
91
55
44
36
35
31
29
26
24
23
23
23
22
22
21
19
18
18
14
14
12
11
10
10
7
7
7
6
4
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
1150
430
338
253
242
231
185
183
173
27
24
21
20
15
13
12
11
9
8
6
6
6
6
5
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4295972
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4296117 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296121 FACS ICC IF IHC (fro) IHC (p) DyLight 650 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296120 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 700 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296123 FACS ICC IF IHC (fro) IHC (p) DyLight 488 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296122 FACS ICC IF IHC (fro) IHC (p) DyLight 680 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296125 FACS ICC IF IHC (fro) IHC (p) DyLight 550 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296127 FACS ICC IF IHC (fro) IHC (p) DyLight 405 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296126 FACS ICC IF IHC (fro) IHC (p) DyLight 350 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296124 FACS ICC IF IHC (fro) IHC (p) DyLight 755 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296129 ICC IF IHC (fro) IHC (p) Biotin Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296119 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 647 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296118 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 405 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4295971 CyTOF Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7

Top referenced anti-CD8 antibody for Immunofluorescence

  • Mouse (Murine) Monoclonal CD8 Primary Antibody for CyTOF, Dep - ABIN4295972 (7 Publications): Fazio, Zappulla, Notartomaso, Busceti, Bessede, Scarselli, Vacca, Gargaro, Volpi, Allegrucci, Lionetto, Simmaco, Belladonna, Nicoletti, Fallarino: Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice. in Neuropharmacology 2014 (PubMed)

Similar anti-CD8 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 4 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Flow Cytometry (FACS) 106 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoaffinity Chromatography (IAC) 2 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 44 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(567), (511), (134), (95), (44), (28), (19), (14), (13), (13), (12), (10), (7), (4), (2), (2), (1), (1)
Host Rat
(739), (482), (186), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(158), (152), (91), (55), (44), (36), (35), (31), (29), (26), (24), (23), (23), (23), (22), (22), (21), (19), (18), (18), (14), (14), (12), (11), (10), (10), (7), (7), (7), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
(1150), (430), (338), (253), (242), (231), (185), (183), (173), (27), (24), (21), (20), (15), (13), (12), (11), (9), (8), (6), (6), (6), (6), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN4295972) Images
Purification Protein A or G purified
Immunogen Mouse thymus or spleen.
Clone 53-6-7
Isotype IgG2a kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN4295972) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 27 kDa
Gene ID 925
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN4295972) Images back to top
Application Notes Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Cell depletion, Inhibition of T Cell Function, CyTOF-readyEach lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 μg/10^6 cells in 100 μL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN4295972) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 Antibody (ABIN4295972)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Li, Wu, Tang, Li, Feng, Yao: "In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice." in: Cancer biotherapy & radiopharmaceuticals, Vol. 31, Issue 9, pp. 317-323, 2016 (Sample species: Human).

Fazio, Zappulla, Notartomaso, Busceti, Bessede, Scarselli, Vacca, Gargaro, Volpi, Allegrucci, Lionetto, Simmaco, Belladonna, Nicoletti, Fallarino: "Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice." in: Neuropharmacology, Vol. 81, pp. 237-43, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kheradmand, Wang, Gibly, Zhang, Holland, Tasch, Graham, Kaufman, Miller, Shea, Luo: "Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions." in: Biomaterials, Vol. 32, Issue 20, pp. 4517-24, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Bankoti, Burnett, Navarro, Miller, Rase, Shepherd: "Effects of TCDD on the fate of naive dendritic cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 115, Issue 2, pp. 422-34, 2010

McLellan, Kapp, Eggert, Linden, Bommhardt, Bröcker, Kämmerer, Kämpgen: "Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression." in: Blood, Vol. 99, Issue 6, pp. 2084-93, 2002

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN4295972) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine s...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha e...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of C...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blo...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of splenocyt...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marr...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen s...