anti-Rat (Rattus) CD8 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine), Rat (Rattus)
598
494
126
92
44
26
19
13
13
13
12
12
9
5
5
5
4
4
4
2
2
1
1
Host
Rat
764
457
197
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
150
143
91
55
45
36
36
33
29
27
24
23
23
23
23
22
20
19
19
18
14
13
11
11
11
10
9
7
7
7
5
4
4
4
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
1148
445
363
254
245
234
196
184
172
35
22
20
16
13
11
11
9
7
6
6
6
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4295972
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967471 BR IAC IHC (fro) FACS IHC (zinc) IHC (p) IP WB Mouse IgG1, kappa Log in to see OX-8 16
1 ABIN4295977 BP CyTOF FACS IHC IHC (fro) IHC (p) IP Neut WB Mouse IgG1 Hinge-like Domain Log in to see OX-8 5
1 ABIN2472531 FACS IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see OX-8 4
1 ABIN2689435 BR IAC IHC (f) IHC (fro) FACS IHC (zinc) IHC (p) IP WB Mouse IgG1, kappa Log in to see OX 10
1 ABIN307936 FACS IHC (fro) IHC (p) Biotin Mouse IgG1 Log in to see MRC OX-8
1 ABIN609286 IHC (fro) FACS IHC (p) Mouse IgG2b Log in to see OX8-2B
1 ABIN4262084 FACS IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262086 FACS IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262085 FACS IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262092 FACS IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262093 FACS IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262089 FACS IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262090 FACS IHC (fro) IHC (p) WB DyLight 550 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262079 FACS IHC (fro) IHC (p) FITC Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262082 FACS IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262083 FACS IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262087 FACS IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262091 FACS IHC (fro) IHC (p) WB DyLight 650 Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN4262081 IHC (fro) IHC (p) WB Biotin Mouse IgG1 Hinge-like Domain Log in to see OX-8
1 ABIN449137 FACS IHC (fro) IHC Mouse IgG1 Log in to see 15-11C5

Top referenced anti-CD8 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD8 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 4 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Flow Cytometry (FACS) 98 Antibodies
Functional Studies (Func) 4 Antibodies
Immunoaffinity Chromatography (IAC) 2 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 44 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(598), (494), (126), (92), (44), (26), (19), (13), (13), (13), (12), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rat
(764), (457), (197), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(150), (143), (91), (55), (45), (36), (36), (33), (29), (27), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (14), (13), (11), (11), (11), (10), (9), (7), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
(1148), (445), (363), (254), (245), (234), (196), (184), (172), (35), (22), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN4295972) Images
Purification Protein A or G purified
Immunogen Mouse thymus or spleen.
Clone 53-6-7
Isotype IgG2a kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN4295972) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 27 kDa
Gene ID 925
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling ProductDetails: References for anti-CD8 antibody (ABIN4295972) Images back to top
Application Notes Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Cell depletion, Inhibition of T Cell Function, CyTOF-readyEach lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 μg/10^6 cells in 100 μL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details ProductDetails: References for anti-CD8 antibody (ABIN4295972) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-CD8 antibody (ABIN4295972)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Cohen, Shani, Raz, Sharon, Hoffman, Abramovitz, Erez: "Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1." in: Oncogene, Vol. 36, Issue 31, pp. 4457-4468, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Li, Wu, Tang, Li, Feng, Yao: "In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice." in: Cancer biotherapy & radiopharmaceuticals, Vol. 31, Issue 9, pp. 317-323, 2016 (Sample species: Human).

Fazio, Zappulla, Notartomaso, Busceti, Bessede, Scarselli, Vacca, Gargaro, Volpi, Allegrucci, Lionetto, Simmaco, Belladonna, Nicoletti, Fallarino: "Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice." in: Neuropharmacology, Vol. 81, pp. 237-43, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kheradmand, Wang, Gibly, Zhang, Holland, Tasch, Graham, Kaufman, Miller, Shea, Luo: "Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions." in: Biomaterials, Vol. 32, Issue 20, pp. 4517-24, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Bankoti, Burnett, Navarro, Miller, Rase, Shepherd: "Effects of TCDD on the fate of naive dendritic cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 115, Issue 2, pp. 422-34, 2010

McLellan, Kapp, Eggert, Linden, Bommhardt, Bröcker, Kämmerer, Kämpgen: "Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression." in: Blood, Vol. 99, Issue 6, pp. 2084-93, 2002

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN4295972) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine s...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha e...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of C...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blo...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of splenocyt...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marr...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen s...