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anti-Sheep (Ovine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Sheep (Ovine)
1059
532
167
68
47
40
25
22
20
19
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to PE
259
216
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1617
452
356
343
285
282
255
238
214
37
33
25
23
17
14
10
7
6
6
6
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN181945
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30464333 ABIN317440 FACS FITC Mouse IgG2a Log in to see CC63 5
0.30464333 ABIN2472467 FACS PE Mouse IgG2a Log in to see CC63 4
0.30464333 ABIN2441211 FACS PE Mouse IgG2a Log in to see
0.30464333 ABIN2472510 FACS PE Mouse IgG2a Log in to see 38-65 4
0.30464333 ABIN2441210 FACS FITC Mouse IgG2a Log in to see
0.0010463598 ABIN181943 FACS FITC Mouse IgG2a Log in to see 38-65 5
0.0010463598 ABIN317442 FACS PE Mouse IgG2a Log in to see CC63 5
0.0010463598 ABIN1449140 IHC (fro) FACS IF IHC (p) IP Mouse IgG2a Log in to see CC63 7
0.0010463598 ABIN290748 FACS FITC Mouse IgG2a Log in to see
0.0010463598 ABIN576488 FACS PE Mouse IgG2a Log in to see
0.0010463598 ABIN2228854 FACS PE Mouse IgG2a Log in to see 10B1988
0.0010463598 ABIN2228853 FACS FITC Mouse IgG2a Log in to see 10B1988
0.0010463598 ABIN2472465 FACS Alexa Fluor 647 Mouse IgG2a Log in to see CC63 4
0.0010463598 ABIN2472466 FACS FITC Mouse IgG2a Log in to see CC63 4
0.0010463598 ABIN2228852 FACS IP IHC Mouse IgG2a Log in to see 10B1988
0.0010463598 ABIN2472509 FACS FITC Mouse IgG2a Log in to see 38-65 4
0.0010463598 ABIN2472464 IHC (fro) FACS IF IP IHC (p) Mouse IgG2a Log in to see CC63 4
0.0010463598 ABIN2472508 IHC (fro) FACS IP Mouse IgG2a Log in to see 38-65 4

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Sheep (Ovine)
Flow Cytometry (FACS) 19 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Sheep (Ovine)
(1059), (532), (167), (68), (47), (40), (25), (22), (20), (19), (19), (16), (15), (13), (12), (12), (11), (11), (10), (10), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1285), (512), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to PE
(259), (216), (142), (86), (52), (42), (40), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1617), (452), (356), (343), (285), (282), (255), (238), (214), (37), (33), (25), (23), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181945) Images
Specificity This anitbody recognises the Ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.
Purification Affinity Chromatography on Protein G
Immunogen Ovine efferent lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Clone 38-65
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN181945) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 281060
NCBI Accession NP_776440
UniProt P31783
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN181945) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN181945) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-CD8 Antibody (ABIN181945)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mackay, Beya, Matzinger: "Gamma/delta T cells express a unique surface molecule appearing late during thymic development." in: European journal of immunology, Vol. 19, Issue 8, pp. 1477-83, 1989

Mackay, Maddox, Brandon: "A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset." in: Cellular immunology, Vol. 110, Issue 1, pp. 46-55, 1987

Mackay, Maddox, Brandon: "Three distinct subpopulations of sheep T lymphocytes." in: European journal of immunology, Vol. 16, Issue 1, pp. 19-25, 1986

Mackay, Maddox, Brandon: "Thymocyte subpopulations during early fetal development in sheep." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 5, pp. 1592-9, 1986

Maddox, Mackay, Brandon: "Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies." in: Immunology, Vol. 55, Issue 4, pp. 739-48, 1985

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181945) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (PE) (ABIN181945) Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE CD8:FITC (ABIN18...