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anti-Dog (Canine) CD80 antibody for Flow Cytometry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Reactivity
Dog (Canine), Mouse (Murine)
408
271
50
16
10
9
8
3
2
2
2
Host
Armenian Hamster
380
109
99
73
49
17
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
78
76
51
26
17
15
15
11
11
10
7
7
6
6
6
6
6
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
488
207
116
109
102
66
46
43
33
22
17
16
14
7
7
7
6
5
5
4
2
2
2
1
1
1
1
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Catalog No. ABIN1176972
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30391768 ABIN2679439 FACS PE-Cy7 Armenian Hamster IgG Log in to see 16-10A1
0.30391768 ABIN2679441 FACS PerCP-eFluor® 710 Armenian Hamster IgG Log in to see 16-10A1
0.30391768 ABIN2679443 FACS EFluor 450 Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN567985 Func IHC (fro) FACS IP Hamster IgG2 Log in to see 16-10A1 9
0.0010469793 ABIN489934 Func FACS IHC (fro) IP Hamster IgG2 Log in to see 16-10A1 8
0.0010469793 ABIN2452902 FACS IF WB Mouse IgG1 Log in to see 1E9
0.0010469793 ABIN2689413 FACS Biotin Armenian Hamster IgG2, kappa Log in to see 16 5
0.0010469793 ABIN282273 Func FACS Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN2689407 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16 6
0.0010469793 ABIN280500 Func FACS IHC (fro) IP Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN282010 FACS PE-Cy5 Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN282744 FACS APC Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN277881 FACS FITC Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN278595 FACS PE Armenian Hamster IgG Log in to see 16-10A1
0.0010469793 ABIN3200026 FACS Func IHC (fro) IP Armenian Hamster IgG2 Log in to see 16-10A1
0.0010469793 ABIN3037606 Func FACS IP IHC Hamster IgG2 Log in to see

Top referenced anti-CD80 antibody for Flow Cytometry

Similar anti-CD80 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
Blocking Reagent (BR) 6 Antibodies
Depletion (Dep) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 21 Antibodies
ELISA (Capture) 4 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 220 Antibodies
Functional Studies (Func) 21 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 56 Antibodies

General

Antigen CD80 Antibodies
Reactivity Dog (Canine), Mouse (Murine)
(408), (271), (50), (16), (10), (9), (8), (3), (2), (2), (2)
Host Armenian Hamster
(380), (109), (99), (73), (49), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(78), (76), (51), (26), (17), (15), (15), (11), (11), (10), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(488), (207), (116), (109), (102), (66), (46), (43), (33), (22), (17), (16), (14), (7), (7), (7), (6), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN1176972) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse CD80 (B7) Transfected Cell Line
Clone 16-10A1
Isotype IgG2, kappa

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN1176972) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background The 16-10A1 antibody reacts with CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4). CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages, and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines. However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4). Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat. No. 553368). In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN-gamma-activated alveolar macrophages of the dog.
Synonyms: B7-1
Research Area Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN1176972) Images back to top
Application Notes Flow Cytometry: For flow cytometry of leukocytes, it is recommended that Mouse BD Fc Block™ (Cat. No. 553141) be used. When using Mouse BD Fc Block™ (clone 2.4G2), it is important that the second-step anti-hamster IgG antibody does not cross-react with the 2.4G2 mAb (rat IgG2b, kappa), we have found that the anti-hamster IgG cocktail (Cat. No. 554010) to be effective. Since this antigen is expressed at low density, it may be desirable to amplify staining by using a biotinylated second-step reagent followed by a bright third-step reagent, such as PE streptavidin (Cat. No. 554061). Expression of membrane CD80 by mouse splenocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141) and stained with either purified 16-10A1 mAb (open histograms) or purified hamster IgG isotype control mAb (shaded histograms). Staining was detected with biotinylated mouse anti-hamster IgG (Cat. No. 554010) followed by PE streptavidin (Cat. No. 554061). Flow cytometry was performed on a FACScan™ (BD Biosciences, San Jose, CA).
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN1176972) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD80 Antibody (ABIN1176972)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Sojka, Donepudi, Bluestone, Mokyr: "Melphalan and other anticancer modalities up-regulate B7-1 gene expression in tumor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 12, pp. 6230-6, 2000

Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, 1997

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

Boussiotis, Gribben, Freeman, Nadler: "Blockade of the CD28 co-stimulatory pathway: a means to induce tolerance." in: Current opinion in immunology, Vol. 6, Issue 5, pp. 797-807, 1995

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994

Razi-Wolf, Freeman, Galvin, Benacerraf, Nadler, Reiser: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, 1992

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN1176972) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 antibody (CD80) (ABIN1176972) anti-CD80 (CD80) antibody