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anti-Human CD80 antibody for Functional Studies

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Reactivity
Human
407
272
50
17
10
9
8
3
2
2
2
Host
Mouse
379
109
99
73
50
17
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
78
76
51
26
17
15
15
11
11
10
7
7
6
6
6
6
6
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
488
207
116
110
102
66
46
43
32
22
17
16
14
7
7
7
7
5
5
4
2
2
2
1
1
1
1
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Catalog No. ABIN111250
€ 675.71
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008762794 ABIN264293 Func IP Mouse IgG1 Extracellular Domain Log in to see AS29
0.0008762794 ABIN609786 Func FACS ELISA WB FITC Mouse IgM Log in to see BB1
0.0008762794 ABIN609785 Func FACS ELISA WB Biotin Mouse IgM Log in to see BB1
0.0008762794 ABIN609784 Func FACS ELISA WB Mouse IgM Log in to see BB1
0.0008762794 ABIN609787 Func FACS ELISA WB Mouse IgM Log in to see BB1
0.0008762794 ABIN282276 Func FACS Mouse IgG1 kappa Log in to see 2D10-4
0.0008762794 ABIN4255214 FACS Func FITC Mouse IgG1 kappa Log in to see 2D10-4
0.0008762794 ABIN4255215 FACS Func FITC Mouse IgG1 kappa Log in to see 2D10-4
0.0008762794 ABIN786608 Func FACS Alexa Fluor 647 Mouse IgG1 Log in to see mAb104 3
0.0008762794 ABIN4255193 FACS Func Mouse IgG1 kappa Log in to see 2D10-4
0.0008762794 ABIN4255194 FACS Func Mouse IgG1 kappa Log in to see 2D10-4

Top referenced anti-CD80 antibody for Functional Studies

Similar anti-CD80 Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 96 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 231 Antibodies
Functional Studies (Func) 12 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 59 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 53 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Neutralization (Neut) 16 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 179 Antibodies

General

Antigen CD80 Antibodies
Reactivity Human
(407), (272), (50), (17), (10), (9), (8), (3), (2), (2), (2)
Host Mouse
(379), (109), (99), (73), (50), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(78), (76), (51), (26), (17), (15), (15), (11), (11), (10), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(488), (207), (116), (110), (102), (66), (46), (43), (32), (22), (17), (16), (14), (7), (7), (7), (7), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN111250) Images
Specificity The antibody reacts with in vitro activated normal B cells. Reacts weakly with a small proportion of non-activated normal B cells. Does not react with peripheral monocytes, resting and activated normal T cells, T-cell lines and T-cell clones.
Purification Protein A affinity chromatography
Immunogen Jijoye cells (Human Burkitt Lymphoma cell line)
Clone MAB104
Isotype IgG1

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN111250) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 941
NCBI Accession NP_005182
UniProt P33681
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN111250) Images back to top
Application Notes Flow cytometry. Functional study of B cell subpopulations. Fluorescence microscopy or flow cytometry (2 g/5x10e5 cells/test). Immunohistochemistry (1: 20 to 1: 50, suitable on frozen sections or cell smears).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN111250) Images back to top
Reconstitution Restore with 1.0 mL of distilled water.
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer PBS containing 1.0 mg/mL BSA
Preservative Azide free
Storage 4 °C
Storage Comment Store at 2 - 8 °C.

References for anti-CD80 Antibody (ABIN111250)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Background publications

Vallé, Aubry, Durand, Banchereau: "IL-4 and IL-2 upregulate the expression of antigen B7, the B cell counterstructure to T cell CD28: an amplification mechanism for T-B cell interactions." in: International immunology, Vol. 3, Issue 3, pp. 229-35, 1991

June, Ledbetter, Linsley, Thompson: "Role of the CD28 receptor in T-cell activation." in: Immunology today, Vol. 11, Issue 6, pp. 211-6, 1990

Linsley, Clark, Ledbetter: "T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 13, pp. 5031-5, 1990

Vallé, Garrone, Yssel, Bonnefoy, Freedman, Freeman, Nadler, Banchereau: "mAb 104, a new monoclonal antibody, recognizes the B7 antigen that is expressed on activated B cells and HTLV-1-transformed T cells." in: Immunology, Vol. 69, Issue 4, pp. 531-5, 1990

Freeman, Freedman, Segil, Lee, Whitman, Nadler: "B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 8, pp. 2714-22, 1989

Freedman, Freeman, Horowitz, Daley, Nadler: "B7, a B-cell-restricted antigen that identifies preactivated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 10, pp. 3260-7, 1987