anti-Human CD80 antibody for Functional Studies

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • CD80 molecule
  • CD8a molecule
  • CD80 antigen
  • Cd80 molecule
  • CD80
  • CD8A
  • Cd80
Epitope
Extracellular Domain
35
24
16
15
15
15
13
11
10
10
8
8
6
5
5
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
460
289
47
11
10
7
6
3
2
2
1
1
Host
Mouse
416
115
101
98
41
20
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
79
75
54
27
21
19
16
14
13
12
11
11
8
8
8
8
7
7
7
5
5
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Immunoprecipitation (IP)
547
206
142
137
121
81
65
44
32
29
26
20
12
10
8
6
6
5
5
4
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN264293
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN609785 Func FACS ELISA WB Biotin Mouse IgM Log in to see BB1
1 ABIN609786 Func FACS ELISA WB FITC Mouse IgM Log in to see BB1
1 ABIN609784 Func FACS ELISA WB Mouse IgM Log in to see BB1
1 ABIN609787 Func FACS ELISA WB Mouse IgM Log in to see BB1
1 ABIN4255193 FACS Func Mouse IgG1 kappa Log in to see 2D10-4
1 ABIN4255194 FACS Func Mouse IgG1 kappa Log in to see 2D10-4
1 ABIN786608 Func FACS Alexa Fluor 647 Mouse IgG1 Log in to see mAb104 3

General

Antigen CD80 Antibodies
Epitope Extracellular Domain
(35), (24), (16), (15), (15), (15), (13), (11), (10), (10), (8), (8), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(460), (289), (47), (11), (10), (7), (6), (3), (2), (2), (1), (1)
Host Mouse
(416), (115), (101), (98), (41), (20)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(79), (75), (54), (27), (21), (19), (16), (14), (13), (12), (11), (11), (8), (8), (8), (8), (7), (7), (7), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Functional Studies (Func), Immunoprecipitation (IP)
(547), (206), (142), (137), (121), (81), (65), (44), (32), (29), (26), (20), (12), (10), (8), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling Images
Specificity Recognizes the extra-cellular domain of human CD80. It does not bind the extracellular domain of CD86.
Characteristics Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1
Purification Protein G Chromatography
Purity > 95 % by SDS-PAGE
Immunogen Recombinant extracellular domain of human CD80.
Clone AS29
Isotype IgG1

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Cell Distribution: CD80 is rapidly induced on the surface of activated B cells, T cells and monocytes and is also expressed at high levels on dendritic cells.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt P33681
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling Images back to top
Application Notes Functional Neutralization: AS29 has been shown to block the interaction of soluble hCD80with hCTLA-4. Immunoprecipitation: This antibody has been used to immunoprecipitate >90 % of CD80 insolution using a double antibody method (Midgley, AR and Hepburn, MR, MethodsEnzymol.70, 266, 1980).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details Images back to top
Buffer PBS without preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months