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|Epitope||Extracellular Domain Alternatives|
|Conjugate||This CD80 antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunoprecipitation (IP)
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Product Details anti-CD80 AntibodyTarget Details CD80 Application Details Handling Images
|Specificity||Recognizes the extra-cellular domain of human CD80. It does not bind the extracellular domain of CD86.|
|Characteristics||Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1|
|Purification||Protein G Chromatography|
|Purity||> 95 % by SDS-PAGE|
|Immunogen||Recombinant extracellular domain of human CD80.|
Target Details CD80Product Details anti-CD80 Antibody Application Details Handling Images back to top
|Alternative Name||CD80 (CD80 Antibody Abstract)|
|Background||CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Cell Distribution: CD80 is rapidly induced on the surface of activated B cells, T cells and monocytes and is also expressed at high levels on dendritic cells.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process|
Application DetailsProduct Details anti-CD80 Antibody Target Details CD80 Handling Images back to top
Functional Neutralization: AS29 has been shown to block the interaction of soluble hCD80with hCTLA-4. Immunoprecipitation: This antibody has been used to immunoprecipitate >90 % of CD80 insolution using a double antibody method (Midgley, AR and Hepburn, MR, MethodsEnzymol.70, 266, 1980).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD80 Antibody Target Details CD80 Application Details Images back to top
|Buffer||PBS without preservatives.|
Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|