anti-Human CD80 antibody for Immunohistochemistry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • CD80 molecule
  • CD8a molecule
  • CD80 antigen
  • Cd80 molecule
  • CD80
  • CD8A
  • Cd80
Epitope
AA 35-242
34
24
17
15
15
15
13
11
10
10
8
8
6
5
5
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
460
289
47
11
10
7
6
3
2
2
1
1
Host
Mouse
416
115
101
98
41
20
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
79
75
54
27
21
19
16
14
13
12
11
11
8
8
8
8
7
7
7
5
5
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut)
546
206
141
137
122
81
65
44
32
30
26
19
11
9
8
6
6
5
5
4
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN4900274
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.452184 ABIN4900275 CyTOF ELISA (Capture) FACS IHC Neut Mouse IgG1 AA 35-242 Log in to see 37711 3
9.452184 ABIN969028 ICC IHC ELISA Mouse IgG1 Log in to see 2A2 2
9.452184 ABIN2453864 IHC WB Mouse IgG2a Log in to see 3F1
9.452184 ABIN2452901 IHC WB Mouse IgG2b Log in to see 2H1
9.452184 ABIN2452899 IF IHC WB Mouse IgG2a Log in to see 2B11
9.452184 ABIN2452900 IF IHC WB Mouse IgG2a Log in to see 2E5
9.452184 ABIN2470886 IF IHC ELISA Mouse IgG1 Log in to see 2A2
9.452184 ABIN4282672 ELISA ICC IF IHC Mouse IgG1 Log in to see 2A2
9.452184 ABIN4255260 IHC Rabbit IgG Log in to see Polyclonal
9.452184 ABIN2432709 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.452184 ABIN2434330 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.452184 ABIN1845393 ELISA IF/ICC IHC Mouse IgG1 Log in to see
1 ABIN4282682 FACS IHC IHC (p) WB Mouse IgG2b kappa Log in to see 62N3G8 1
1 ABIN4903203 FACS IF IHC WB Mouse IgG Log in to see
1 ABIN4282673 ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 2E5
1 ABIN5645592 CyTOF ELISA (Capture) FACS IHC Neut Mouse IgG1 AA 35-242 Log in to see 37711
1 ABIN5645591 CyTOF ELISA (Capture) FACS IHC Neut Mouse IgG1 AA 35-242 Log in to see 37711
1 ABIN2869411 ELISA ICC IHC Mouse IgG1 Log in to see 2A2
1 ABIN4282683 FACS IHC IHC (p) WB Alexa Fluor 700 Mouse IgG2b kappa Log in to see 62N3G8
1 ABIN204040 FACS IHC Biotin Mouse IgG1 Log in to see

General

Antigen CD80 Antibodies
Epitope AA 35-242
(34), (24), (17), (15), (15), (15), (13), (11), (10), (10), (8), (8), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(460), (289), (47), (11), (10), (7), (6), (3), (2), (2), (1), (1)
Host Mouse
(416), (115), (101), (98), (41), (20)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(79), (75), (54), (27), (21), (19), (16), (14), (13), (12), (11), (11), (8), (8), (8), (8), (7), (7), (7), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut)
(546), (206), (141), (137), (122), (81), (65), (44), (32), (30), (26), (19), (11), (9), (8), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900274) Images
Specificity Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80, Val35-Asn242 (predicted), Accession # P33681
Clone 37711
Isotype IgG1

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN4900274) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt P33681
Research Area Stem Cells, Adaptive Immunity, Hematopoietic Progenitors, Surface Receptors of Immune Cells, CD Antigens
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN4900274) Images back to top
Application Notes Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Neutralization 0.2-1 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN4900274) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD80 antibody (ABIN4900274)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Ribot, Debarros, Mancio-Silva, Pamplona, Silva-Santos: "B7-CD28 costimulatory signals control the survival and proliferation of murine and human ?? T cells via IL-2 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 3, pp. 1202-8, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Weissmüller, Semmler, Kalinke, Christians, Müller-Berghaus, Waibler: "ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation." in: Blood, Vol. 119, Issue 26, pp. 6268-77, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Kosaka, Yashiro, Sakate, Hirakawa: "A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma." in: Digestive diseases and sciences, Vol. 52, Issue 8, pp. 1946-53, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN4900274) back to top
Supplier Images
Neutralization (Neut) image for anti-CD80 (CD80) (AA 35-242) antibody (ABIN4900274) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Human B7-1/CD80 Antibody.
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 35-242) antibody (ABIN4900274) Detection of B7-1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt'...
Immunohistochemistry (IHC) image for anti-CD80 (CD80) (AA 35-242) antibody (ABIN4900274) B7-1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedde...
Neutralization (Neut) image for anti-CD80 (CD80) (AA 35-242) antibody (ABIN4900274) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Human B7-1/CD80 Antibody.
Immunohistochemistry (IHC) image for anti-CD80 (CD80) (AA 35-242) antibody (ABIN4900274) B7-1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedde...
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 35-242) antibody (ABIN4900274) Detection of B7-1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt'...