anti-Mouse (Murine) CD80 antibody for ELISA (Capture)

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • CD80 molecule
  • CD8a molecule
  • CD80 antigen
  • Cd80 molecule
  • CD80
  • CD8A
  • Cd80
Epitope
AA 37-245
24
22
17
15
15
15
13
11
10
10
8
8
6
5
5
4
3
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
448
281
47
11
10
7
6
3
2
2
1
1
Host
Goat
404
115
102
91
41
18
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
76
74
53
26
20
18
15
14
13
12
10
10
8
8
8
8
7
7
7
5
5
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
525
204
140
137
122
81
65
44
30
30
26
16
8
8
6
6
6
5
5
4
2
2
2
2
1
1
1
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Catalog No. ABIN4900307
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2661827 ELISA (Capture) WB FITC Hamster IgG Log in to see 16-10A1 11
1 ABIN2659009 CyTOF ELISA (Capture) ELISPOT (Capture) ICC IHC IP Neut PE-Cy5 Hamster IgG Log in to see 16-10A1 11
1 ABIN2661826 ELISA (Capture) WB FITC Hamster IgG Log in to see 16-10A1 13
1 ABIN2659010 CyTOF ELISA (Capture) ELISPOT (Capture) ICC IHC IP Neut PE-Cy5 Hamster IgG Log in to see 16-10A1 12

General

Antigen CD80 Antibodies
Epitope AA 37-245
(24), (22), (17), (15), (15), (15), (13), (11), (10), (10), (8), (8), (6), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(448), (281), (47), (11), (10), (7), (6), (3), (2), (2), (1), (1)
Host Goat
(404), (115), (102), (91), (41), (18)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(76), (74), (53), (26), (20), (18), (15), (14), (13), (12), (10), (10), (8), (8), (8), (8), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(525), (204), (140), (137), (122), (81), (65), (44), (30), (30), (26), (16), (8), (8), (6), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN4900307) Images
Specificity Detects mouse B7-1/CD80 in ELISAs and Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80, Asp37-Lys245, Accession # Q00609
Isotype IgG

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN4900307) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt Q00609
Research Area Stem Cells, Adaptive Immunity, Hematopoietic Progenitors, Surface Receptors of Immune Cells, CD Antigens
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling ProductDetails: References for anti-CD80 antibody (ABIN4900307) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.15-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details ProductDetails: References for anti-CD80 antibody (ABIN4900307) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-CD80 antibody (ABIN4900307)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Sofra, Mansour, Liu, Gao, Primpidou, Wang, Li: "Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection." in: European journal of immunology, Vol. 39, Issue 1, pp. 85-95, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Li, Chen, Zhang, Chen, Hayashida, Zhu, Tseng: "Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract." in: Investigative ophthalmology & visual science, Vol. 49, Issue 10, pp. 4468-75, 2008 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling ProductDetails: References for anti-CD80 antibody (ABIN4900307) back to top
Supplier Images
Neutralization (Neut) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Immunocytochemistry (ICC) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 3)
Western Blotting (WB) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Western Blotting (WB) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...
Neutralization (Neut) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Immunocytochemistry (ICC) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 8)