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anti-Mouse (Murine) CD80 antibody for Immunocytochemistry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • B71
  • BB1
  • CD8
  • Cd28l
  • CD28LG
  • CD28LG1
  • LAB7
  • Leu2
  • Ly-53
  • Ly53
  • MAL
  • MIC17
  • p32
  • TSA1
Epitope
AA 37-245
24
22
16
15
14
14
14
13
11
6
5
5
5
5
3
2
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
409
267
49
10
8
8
3
2
2
2
Host
Goat
377
113
102
71
41
17
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
78
76
49
25
17
15
13
12
11
11
7
7
6
6
6
6
6
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
481
206
119
118
104
63
47
43
30
24
18
17
13
7
7
7
7
6
5
5
4
2
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN4900307
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001010224 ABIN2657475 ICC IF IP IHC ELISA WB Alexa Fluor 488 Hamster IgG Log in to see 16-10A1 11
0.001010224 ABIN2659009 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 16-10A1 11
0.001010224 ABIN2657476 ICC IF IP IHC ELISA WB Alexa Fluor 488 Hamster IgG Log in to see 16-10A1 11
0.001010224 ABIN2659010 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 16-10A1 12
0.001010224 ABIN217937 ICC Rat IgG2a Log in to see
0.001010224 ABIN956674 ICC FACS ELISA WB Goat IgG Log in to see Polyclonal
0.001010224 ABIN1854865 ICC IF IHC WB Mouse IgG2b Extracellular Log in to see
0.001010224 ABIN645569 ICC Rat IgG2a Log in to see

Top referenced anti-CD80 antibody for Immunocytochemistry

Similar anti-CD80 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
Blocking Reagent (BR) 6 Antibodies
Depletion (Dep) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 21 Antibodies
ELISA (Capture) 5 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 211 Antibodies
Functional Studies (Func) 19 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 55 Antibodies

General

Antigen CD80 Antibodies
Epitope AA 37-245
(24), (22), (16), (15), (14), (14), (14), (13), (11), (6), (5), (5), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(409), (267), (49), (10), (8), (8), (3), (2), (2), (2)
Host Goat
(377), (113), (102), (71), (41), (17)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(78), (76), (49), (25), (17), (15), (13), (12), (11), (11), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(481), (206), (119), (118), (104), (63), (47), (43), (30), (24), (18), (17), (13), (7), (7), (7), (7), (6), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN4900307) Images
Specificity Detects mouse B7-1/CD80 in ELISAs and Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 g of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80, Asp37-Lys245, Accession # Q00609
Isotype IgG

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 Antibody (ABIN4900307) Images back to top
Antigen
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Gene ID 941
UniProt Q00609
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 Antibody (ABIN4900307) Images back to top
Application Notes Western Blot 1 μg/mL, Flow Cytometry 0.25 μg/10^6 cells, Neutralization 0.15-0.6 μg/mL, Immunocytochemistry 5-15 μg/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 Antibody (ABIN4900307) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD80 Antibody (ABIN4900307)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Sofra, Mansour, Liu, Gao, Primpidou, Wang, Li: "Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection." in: European journal of immunology, Vol. 39, Issue 1, pp. 85-95, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Li, Chen, Zhang, Chen, Hayashida, Zhu, Tseng: "Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract." in: Investigative ophthalmology & visual science, Vol. 49, Issue 10, pp. 4468-75, 2008 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling References for anti-CD80 Antibody (ABIN4900307) back to top
Supplier Images
Neutralization (Neut) image for anti-CD80 antibody (CD80) (AA 37-245) (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Flow Cytometry (FACS) image for anti-CD80 antibody (CD80) (AA 37-245) (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Immunocytochemistry (ICC) image for anti-CD80 antibody (CD80) (AA 37-245) (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 3)
Western Blotting (WB) image for anti-CD80 antibody (CD80) (AA 37-245) (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...