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anti-Rat (Rattus) CD80 antibody for Immunocytochemistry

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • CD80 molecule
  • CD8a molecule
  • CD80 antigen
  • Cd80 molecule
  • CD80
  • CD8A
  • Cd80
Epitope
Extracellular Domain
24
22
16
15
15
15
13
11
10
10
8
8
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Rat (Rattus)
435
271
46
10
10
7
6
3
2
2
1
Host
Goat
391
114
97
85
41
17
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
75
71
49
25
20
18
15
14
12
11
10
10
8
8
8
8
7
5
5
5
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
510
199
132
125
113
70
62
44
30
28
26
9
8
7
7
7
7
6
5
5
4
2
2
2
2
1
1
1
1
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Catalog No. ABIN956675
$ 793.83
Plus shipping costs $45.00

Similar anti-CD80 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 5 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 12 Antibodies

General

Antigen CD80 Antibodies
Epitope Extracellular Domain
(24), (22), (16), (15), (15), (15), (13), (11), (10), (10), (8), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(435), (271), (46), (10), (10), (7), (6), (3), (2), (2), (1)
Host Goat
(391), (114), (97), (85), (41), (17)
Clonality
Polyclonal
Conjugate This CD80 antibody is un-conjugated
(75), (71), (49), (25), (20), (18), (15), (14), (12), (11), (10), (10), (8), (8), (8), (8), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(510), (199), (132), (125), (113), (70), (62), (44), (30), (28), (26), (9), (8), (7), (7), (7), (7), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling Images
Specificity Rat B7-1 extracellular domain.
Purification Immunoaffinity purified
Sterility 0.2 μm filtered
Immunogen Purified, NS0-derived, recombinant rat B7-1 (rrB7-1) extracellular domain.

Type of Immunogen: Recombinant protein
Isotype IgG

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD80 / B7 (CD80 Antibody Abstract)
Background Name/Gene ID: CD80

Synonyms: CD80, Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CD80 antigen, LAB7, CD80 Molecule, CTLA-4 counter-receptor B7.1
Gene ID 941
UniProt P33681
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling Images back to top
Application Notes Approved: ELISA (0.5 - 1 μg/mL), ICC (5 - 15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect rat B7-1. The detection limit for rrB7-1 is approximately 0.1 ng/well. In this format, this antibody shows approximately 15 % cross-reactivity with rmB7-1, 5 % cross-reactivity with rhB7-1 and less than 1 % cross-reactivity with rmB7-H3, rrB7-2, rmB7-H1 and rmPD-L2. Western blot-This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect rat B7-1. The detection limit for rrB7-1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry-This antibody will detect B7-1 in cells. The working dilution is 5-15 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details Images back to top
Format Lyophilized
Concentration Lot specific
Buffer Lyophilized from 0.2 μm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1 % sucrose.
Storage -20 °C
Storage Comment Store at -20°C.