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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||150 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||19 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||16 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||27 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Western Blotting (WB)||49 Antibodies|
|Conjugate||This CD83 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CD83 AntibodyTarget Details CD83 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of CD83 protein. (region surrounding Pro130)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details CD83Product Details anti-CD83 Antibody Application Details Handling Images back to top
|Alternative Name||CD83 (CD83 Antibody Abstract)|
|Background||CD83 is a heavily glycosylated membrane protein of the immunoglobulin (Ig) superfamily that is expressed in mature dendritic cells, Langerhans cells and interdigitating reticulum cells within lymphoid tissues. Structurally, CD83 resembles other Ig superfamily members, which have an extracellular V-type Ig-like domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 expression is used as a marker for mature, antigen presenting dendritic cells that are capable of generating tumor-specific T cell immunity, a phenotype with implications as an anticancer vaccine. CD83-IgG1(fc) chimera studies indicate that CD83 is a sialic acid-binding, Ig-like lectin (Siglec) adhesion molecule that is involved in cell adhesion/signaling by hosting dendritic cell interactions with monocytes and CD8+ T cells.Synonyms: B-cell activation protein, Cell surface protein HB15, HB15|
|Molecular Weight||approx. 23, 45 kDa|
|Research Area||Immunology, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD83 Antibody Target Details CD83 Handling Images back to top
ELISA: 1/1000approx. 1/5000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD83 Antibody Target Details CD83 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CD83 Antibody Target Details CD83 Application Details Handling back to top