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anti-Human CREB1 antibody for Immunohistochemistry (Frozen Sections)

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • CREB
  • creb
  • Creb-1
  • creb1
  • Creb1
  • CREB1
  • zgc:55598
Epitope
pSer133
109
35
33
28
23
21
17
16
16
13
10
7
7
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
468
230
223
26
24
19
17
14
13
9
9
8
2
1
1
1
Host
Rabbit
404
70
10
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
15
14
13
7
6
6
5
4
3
3
3
3
3
3
3
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
371
216
184
67
58
36
35
28
26
11
9
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN372604
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088847685 ABIN353293 FACS IHC (fro) IF IHC (p) IP WB Rabbit IgG Log in to see

Top referenced anti-CREB1 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CREB1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken Cow (Bovine) Sheep (Ovine) Xenopus laevis Zebrafish (Danio rerio) Non Human Primates
Western Blotting (WB) 362 Antibodies 165 Antibodies 159 Antibodies 14 Antibodies 21 Antibodies 23 Antibodies 16 Antibodies 9 Antibodies 15 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 38 Antibodies 22 Antibodies

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Epitope pSer133
(109), (35), (33), (28), (23), (21), (17), (16), (16), (13), (10), (7), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
(468), (230), (223), (26), (24), (19), (17), (14), (13), (9), (9), (8), (2), (1), (1), (1)
Host Rabbit
(404), (70), (10), (1)
Clonality
Polyclonal
Conjugate This CREB1 antibody is un-conjugated
(15), (14), (13), (7), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(371), (216), (184), (67), (58), (36), (35), (28), (26), (11), (9), (3), (3), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN372604) Images
Specificity Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.
Isotype IgG

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling References for anti-CREB1 Antibody (ABIN372604) Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002).Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 81646
NCBI Accession NP_112279
UniProt P15337
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling References for anti-CREB1 Antibody (ABIN372604) Images back to top
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details References for anti-CREB1 Antibody (ABIN372604) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-CREB1 Antibody (ABIN372604)

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Background publications

Johannessen, Delghandi, Moens: "What turns CREB on?" in: Cellular signalling, Vol. 16, Issue 11, pp. 1211-27, 2004

Kornhauser, Cowan, Shaywitz, Dolmetsch, Griffith, Hu, Haddad, Xia, Greenberg: "CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events." in: Neuron, Vol. 34, Issue 2, pp. 221-33, 2002

Shaywitz, Greenberg: "CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals." in: Annual review of biochemistry, Vol. 68, Issue 2, pp. 821-61, 2000

Silva, Kogan, Frankland, Kida: "CREB and memory." in: Annual review of neuroscience, Vol. 21, pp. 127-48, 1998

Montminy: "Transcriptional regulation by cyclic AMP." in: Annual review of biochemistry, Vol. 66, pp. 807-22, 1997

Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN372604) back to top
Supplier Images
 image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN372604) anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody