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anti-Mouse (Murine) CREB1 antibody for Electrophoretic Mobility-Shift Assay

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • CREB
  • creb
  • Creb-1
  • creb1
  • Creb1
  • CREB1
  • zgc:55598
Epitope
N-Term
110
35
33
28
23
21
17
16
15
13
10
7
7
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
468
230
223
27
25
19
18
15
14
9
9
9
2
2
1
1
Host
Rabbit
404
70
10
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
15
14
13
7
6
6
5
4
3
3
3
3
3
3
3
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
371
216
184
67
58
36
34
28
26
10
9
3
3
2
2
1
1
Supplier
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Catalog No. ABIN116948
$ 500.50
Plus shipping costs $45.00

Top referenced anti-CREB1 antibody for Electrophoretic Mobility-Shift Assay

Similar anti-CREB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies 1 Antibodies
ELISA 71 Antibodies 72 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 114 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 159 Antibodies

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Epitope N-Term
(110), (35), (33), (28), (23), (21), (17), (16), (15), (13), (10), (7), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(468), (230), (223), (27), (25), (19), (18), (15), (14), (9), (9), (9), (2), (2), (1), (1)
Host Rabbit
(404), (70), (10), (1)
Clonality
Polyclonal
Conjugate This CREB1 antibody is un-conjugated
(15), (14), (13), (7), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3)
Application Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(371), (216), (184), (67), (58), (36), (34), (28), (26), (10), (9), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN116948) Images
Specificity This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Immunogen CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling References for anti-CREB1 Antibody (ABIN116948) Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 1385
NCBI Accession NP_004370
UniProt P16220
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling References for anti-CREB1 Antibody (ABIN116948) Images back to top
Application Notes Western Blot: This antibody was assayed against a lysate of 1 x 10^6 Raji B cells byImmunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at adilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG(H&L) [Goat] (Cat#R1364HRP)ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gelshift: This antibody was tested in Gel Supershift Assay and found to be reactive againstCREB-1 (p43) using 0.5-1.0 μL per assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details References for anti-CREB1 Antibody (ABIN116948) Images back to top
Format Liquid
Concentration 2.56 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

References for anti-CREB1 Antibody (ABIN116948)

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Background publications

Moreno, Beresford, Louis-Plence, Morris, Boss: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN116948) back to top
Supplier Images
Western Blotting (WB) image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (N-Term) (ABIN116948) Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract lysates. D...