anti-Mouse (Murine) CREB1 antibody for Immunocytochemistry

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Reactivity
Human, Mouse (Murine), Rat (Rattus), Monkey
548
328
298
41
38
36
30
26
26
26
19
17
15
12
7
7
7
2
1
1
1
1
Host
Mouse
450
100
11
1
Clonality
Monoclonal
Conjugate
This CREB1 antibody is un-conjugated
18
15
15
7
7
6
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
441
224
191
100
92
47
43
42
31
11
11
10
4
3
3
2
2
1
1
1
Options
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Catalog No. ABIN969061
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.04059 ABIN261497 ICC IF IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see LB9 1
1 ABIN257735 ICC IF IHC (p) IP IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal
1 ABIN1497628 ELISA ICC IF IHC (p) WB Mouse IgG1 Log in to see 5G3
1 ABIN1871996 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4300443 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 5G3
1 ABIN294690 ICC IF IHC (p) ELISA WB Rabbit pSer133 Log in to see Polyclonal
1 ABIN4300445 ChIP ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4300441 ICC IF IHC IHC (p) Rabbit pSer129 Log in to see Polyclonal
1 ABIN4300444 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN2740072 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN4300440 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4880261 ELISA ICC IHC WB Mouse IgG1 Log in to see 5G3
1 ABIN1964669 ELISA FACS ICC IF WB APC Rabbit IgG AA 112-140 Log in to see Polyclonal
1 ABIN1962290 ELISA FACS ICC IF WB Alkaline Phosphatase (AP) Rabbit IgG AA 112-140 Log in to see Polyclonal
1 ABIN1971097 ELISA FACS ICC IF WB FITC Rabbit IgG AA 112-140 Log in to see Polyclonal
1 ABIN1968793 ELISA FACS ICC IF WB Biotin Rabbit IgG AA 112-140 Log in to see Polyclonal
1 ABIN1975681 ELISA FACS ICC IF WB PE Rabbit IgG AA 112-140 Log in to see Polyclonal
1 ABIN1973711 ELISA FACS ICC IF WB HRP Rabbit IgG AA 112-140 Log in to see Polyclonal
1 ABIN227649 ICC IHC (p) IP ELISA WB Rabbit IgG pSer133 Log in to see Polyclonal
1 ABIN4903372 FACS ICC IF IHC IP WB Rabbit IgG Log in to see

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus), Monkey
(548), (328), (298), (41), (38), (36), (30), (26), (26), (26), (19), (17), (15), (12), (7), (7), (7), (2), (1), (1), (1), (1)
Host Mouse
(450), (100), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CREB1 antibody is un-conjugated
(18), (15), (15), (7), (7), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(441), (224), (191), (100), (92), (47), (43), (42), (31), (11), (11), (10), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling ProductDetails: References for anti-CREB1 antibody (ABIN969061) Images
Purification Ascites
Immunogen Purified recombinant fragment of human CREB1 expressed in E. Coli.
Clone 5G3
Isotype IgG1

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling ProductDetails: References for anti-CREB1 antibody (ABIN969061) Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)
Molecular Weight 43 kDa
Gene ID 1385
HGNC 1385
Pathways TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling ProductDetails: References for anti-CREB1 antibody (ABIN969061) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details ProductDetails: References for anti-CREB1 antibody (ABIN969061) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

ProductDetails: References for anti-CREB1 antibody (ABIN969061)

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Product cited in:

Jansson, Ng, Fu, Depatie, Al Azzabi, Screaton: "Glucose controls CREB activity in islet cells via regulated phosphorylation of TORC2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 29, pp. 10161-6, 2008

Mamdani, Alda, Grof, Young, Rouleau, Turecki: "Lithium response and genetic variation in the CREB family of genes." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 147B, Issue 4, pp. 500-4, 2008

Seo, Chung: "CREB activates proteasomal degradation of DSCR1/RCAN1." in: FEBS letters, Vol. 582, Issue 13, pp. 1889-93, 2008

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling ProductDetails: References for anti-CREB1 antibody (ABIN969061) back to top
Supplier Images
ELISA image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Immunocytochemistry (ICC) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 2: Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green) . R...
Immunohistochemistry (IHC) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (...
Western Blotting (WB) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 4: Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L...
'Independent Validation' Badge
Antigen CREB1
Lot Number 110517
Method validated Western Blotting
Positive Control primary mouse microglia
Negative Control siRNA knock-down of CREB1 in primary mouse microglia
Notes Passed. ABIN969061 specifically recognizes Creb1 in primary mouse microglia lysates.
Primary Antibody ABIN969061
Secondary Antibody anti-mouse HRP-conjugated antibody (DakoCytomation, P0447, lot 00058737)
Protocol
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse Creb1 Silencer Select siRNA (ThermoFisher Scientific, ASO1093M, ASO10982 and ASO109CG, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, containing protease inhibitors (Sigma)).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg of total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary mouse anti-CREB1 antibody (antibodies-online, ABIN969061, lot 110517) diluted 1:10000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-mouse horseradish peroxidase-conjugated secondary antibody (DakoCytomation, P0447, lot 00058737) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the membrane as described above.
Experimental Notes The CREB1 antibody ABIN969061 reveals a protein of the expected molecular weight of murine Creb1 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validation Images
Western Blotting validation image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Primary mouse microglia were transfected with Creb1 siRNA and Creb1 was revealed usin...