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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||361 Antibodies||162 Antibodies||158 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||38 Antibodies||21 Antibodies||19 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||61 Antibodies||29 Antibodies||29 Antibodies|
|Immunohistochemistry (IHC)||218 Antibodies||113 Antibodies||117 Antibodies|
|Antigen||CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CREB1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-CREB1 AntibodyTarget Details CREB1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of CREB-1 protein only when phosphorylated at Ser129.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details CREB1Product Details anti-CREB1 Antibody Application Details Handling Images back to top
|Alternative Name||CREB1 (CREB1 Antibody Abstract)|
|Background||Eukaryotic gene transcription is regulated by sequencespecific transcription factors that bind modular CIS acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a Cterminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser 133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1|
|Molecular Weight||approx. 38 kDa|
|Pathways||TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CREB1 Antibody Target Details CREB1 Handling Images back to top
Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling back to top