anti-Rat (Rattus) CREB1 antibody for Enzyme Immunoassay

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Epitope
N-Term
142
38
38
37
22
20
15
15
15
9
8
7
7
6
6
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
548
328
298
41
38
36
30
27
26
26
19
17
15
12
7
7
7
2
1
1
1
1
Host
Rabbit
449
101
11
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
18
15
15
7
7
6
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
441
225
192
100
92
46
44
42
31
11
10
10
4
3
3
2
2
1
1
Supplier
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Catalog No. ABIN116948
$ 419.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN265371 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5556654 EIA IP WB Rabbit pSer133 Log in to see Polyclonal
1 ABIN1106831 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5G3 4

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Epitope N-Term
(142), (38), (38), (37), (22), (20), (15), (15), (15), (9), (8), (7), (7), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(548), (328), (298), (41), (38), (36), (30), (27), (26), (26), (19), (17), (15), (12), (7), (7), (7), (2), (1), (1), (1), (1)
Host Rabbit
(449), (101), (11), (1)
Clonality
Polyclonal
Conjugate This CREB1 antibody is un-conjugated
(18), (15), (15), (7), (7), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(441), (225), (192), (100), (92), (46), (44), (42), (31), (11), (10), (10), (4), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling ProductDetails: References for anti-CREB1 antibody (ABIN116948) Images
Specificity This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Immunogen CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling ProductDetails: References for anti-CREB1 antibody (ABIN116948) Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 1385
NCBI Accession NP_004370
UniProt P16220
Pathways TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling ProductDetails: References for anti-CREB1 antibody (ABIN116948) Images back to top
Application Notes Western Blot: This antibody was assayed against a lysate of 1 x 10^6 Raji B cells byImmunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at adilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG(H&L) [Goat] (Cat#R1364HRP)ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gelshift: This antibody was tested in Gel Supershift Assay and found to be reactive againstCREB-1 (p43) using 0.5-1.0 μL per assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details ProductDetails: References for anti-CREB1 antibody (ABIN116948) Images back to top
Format Liquid
Concentration 2.56 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

ProductDetails: References for anti-CREB1 antibody (ABIN116948)

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Background publications

Moreno, Beresford, Louis-Plence, Morris, Boss: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling ProductDetails: References for anti-CREB1 antibody (ABIN116948) back to top
Supplier Images
Western Blotting (WB) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) (N-Term) antibody (ABIN116948) Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract lysates. D...