|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)||Chicken||Cow (Bovine)||Sheep (Ovine)||Xenopus laevis||Zebrafish (Danio rerio)||Non Human Primates|
|Western Blotting (WB)||387 Antibodies||189 Antibodies||172 Antibodies||14 Antibodies||21 Antibodies||23 Antibodies||16 Antibodies||9 Antibodies||15 Antibodies||2 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||50 Antibodies||35 Antibodies|
|Antigen||CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This CREB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|6 references available|
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Product Details anti-CREB1 AntibodyTarget Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN372604) Images
|Specificity||Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.|
Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.|
Target Details CREB1Product Details anti-CREB1 Antibody Application Details Handling References for anti-CREB1 Antibody (ABIN372604) Images back to top
|Alternative Name||CREB1 (CREB1 Antibody Abstract)|
|Background||It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002).Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1|
|Pathways||TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CREB1 Antibody Target Details CREB1 Handling References for anti-CREB1 Antibody (ABIN372604) Images back to top
Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details References for anti-CREB1 Antibody (ABIN372604) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-CREB1 Antibody (ABIN372604)Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Johannessen, Delghandi, Moens: "What turns CREB on?" in: Cellular signalling, Vol. 16, Issue 11, pp. 1211-27, 2004
Kornhauser, Cowan, Shaywitz, Dolmetsch, Griffith, Hu, Haddad, Xia, Greenberg: "CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events." in: Neuron, Vol. 34, Issue 2, pp. 221-33, 2002
Shaywitz, Greenberg: "CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals." in: Annual review of biochemistry, Vol. 68, Issue 2, pp. 821-61, 2000
Silva, Kogan, Frankland, Kida: "CREB and memory." in: Annual review of neuroscience, Vol. 21, pp. 127-48, 1998
Montminy: "Transcriptional regulation by cyclic AMP." in: Annual review of biochemistry, Vol. 66, pp. 807-22, 1997
Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995
ImagesProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling References for anti-CREB1 Antibody (ABIN372604) back to top