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anti-Human Crk antibody for Enzyme Immunoassay

Recommended Crk Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies
  • c-Crk
  • CG1587
  • crk
  • CRK
  • Crk-I
  • Crk-II
  • Crk-III
  • crk2
  • Crk3
  • crkII
  • CRKII
  • CrkIII
  • Crko
  • D-CRK
  • D-Crk
  • dCrk
  • Dcrk
  • dCRK
  • DCrk
  • Dmel\\CG1587
  • fb34h10
  • fc05a01
  • fi19g05
  • p38
  • P38C-CRK
  • v-crk
  • wu:fb34h10
  • wu:fc05a01
  • wu:fc54a04
  • wu:fi19g05
  • zgc:100936
Epitope
pSer41
23
15
12
6
5
4
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
130
61
55
3
2
2
2
2
2
1
Host
Rabbit
97
33
Clonality
Polyclonal
Conjugate
This Crk antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA)
80
45
26
24
22
22
18
15
12
4
3
2
2
Supplier
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Catalog No. ABIN358272
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN265373 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008867729 ABIN1106834 EIA IF IHC (p) WB Mouse IgG2b Log in to see 3G11C1 2
0.0008867729 ABIN1106835 EIA IF IHC (p) WB Mouse IgG2b Log in to see 3G11E8 2

Top referenced anti-Crk antibody for Enzyme Immunoassay

Similar anti-Crk Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 22 Antibodies
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies
Epitope pSer41
(23), (15), (12), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(130), (61), (55), (3), (2), (2), (2), (2), (2), (1)
Host Rabbit
(97), (33)
Clonality
Polyclonal
Conjugate This Crk antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA)
(80), (45), (26), (24), (22), (22), (18), (15), (12), (4), (3), (2), (2)
Pubmed 5 references available
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Product Details anti-Crk Antibody

Target Details Crk Application Details Handling References for anti-Crk Antibody (ABIN358272) Images
Specificity This antibody detects CRK pSer41. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S41 of human CRK.
Isotype Ig

Target Details Crk

Product Details anti-Crk Antibody Application Details Handling References for anti-Crk Antibody (ABIN358272) Images back to top
Antigen
Alternative Name CRK (CRK Antibody Abstract)
Background CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38
Molecular Weight 22906 Da
Gene ID 1398
NCBI Accession NP_005197
UniProt P46108
Pathways TCR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Crk Antibody Target Details Crk Handling References for anti-Crk Antibody (ABIN358272) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Crk Antibody Target Details Crk Application Details References for anti-Crk Antibody (ABIN358272) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Crk Antibody (ABIN358272)

Product Details anti-Crk Antibody Target Details Crk Application Details Handling Images back to top
Background publications

Bougnères, Girardin, Weed, Karginov, Olivo-Marin, Parsons, Sansonetti, Van Nhieu: "Cortactin and Crk cooperate to trigger actin polymerization during Shigella invasion of epithelial cells." in: The Journal of cell biology, Vol. 166, Issue 2, pp. 225-35, 2004

Stoletov, Gong, Terman: "Nck and Crk mediate distinct VEGF-induced signaling pathways that serve overlapping functions in focal adhesion turnover and integrin activation." in: Experimental cell research, Vol. 295, Issue 1, pp. 258-68, 2004

Miller, Chen, Gharib, Wang, Thomas, Misek, Giordano, Yee, Orringer, Hanash, Beer: "Increased C-CRK proto-oncogene expression is associated with an aggressive phenotype in lung adenocarcinomas." in: Oncogene, Vol. 22, Issue 39, pp. 7950-7, 2003

Sun, Barbieri: "Pseudomonas aeruginosa ExoT ADP-ribosylates CT10 regulator of kinase (Crk) proteins." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 32794-800, 2003

Zhang, He, Zhou, Liu: "Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27319-28, 2003

Images

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Supplier Images
 image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (pSer41) (ABIN358272) anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (pSer41) antibody