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|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|Dot Blot (DB)||22 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Immunocytochemistry (ICC)||17 Antibodies|
|Immunofluorescence (IF)||25 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||22 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||78 Antibodies|
|Antigen||V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies|
|Conjugate||This Crk antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-Crk AntibodyTarget Details Crk Application Details Handling References for anti-Crk Antibody (ABIN969062) Images
|Immunogen||Purified recombinant fragment of human CRK expressed in E. coli.|
Target Details CrkProduct Details anti-Crk Antibody Application Details Handling References for anti-Crk Antibody (ABIN969062) Images back to top
|Alternative Name||CRK (CRK Antibody Abstract)|
|Background||This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.|
|Molecular Weight||42 kDa|
|Pathways||TCR Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-Crk Antibody Target Details Crk Handling References for anti-Crk Antibody (ABIN969062) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Crk Antibody Target Details Crk Application Details References for anti-Crk Antibody (ABIN969062) Images back to top
|Buffer||Ascitic fluid containing 0.03 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-Crk Antibody (ABIN969062)Product Details anti-Crk Antibody Target Details Crk Application Details Handling Images back to top
|Product cited in:||
Watanabe, Tsuda, Tanaka et al.: "Adaptor protein Crk induces Src-dependent activation of p38 MAPK in regulation of synovial sarcoma cell proliferation." in: Molecular cancer research : MCR, Vol. 7, Issue 9, pp. 1582-92, 2009 (PubMed).
Tanaka: "[Biological roles of signaling adaptor protein CRK]." in: Seikagaku. The Journal of Japanese Biochemical Society, Vol. 81, Issue 5, pp. 361-76, 2009 (PubMed).
ImagesProduct Details anti-Crk Antibody Target Details Crk Application Details Handling References for anti-Crk Antibody (ABIN969062) back to top