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anti-Human Crk antibody for Immunohistochemistry

Recommended Crk Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies
  • c-Crk
  • CG1587
  • crk
  • CRK
  • Crk-I
  • Crk-II
  • Crk-III
  • crk2
  • Crk3
  • crkII
  • CRKII
  • CrkIII
  • Crko
  • D-CRK
  • D-Crk
  • dCrk
  • Dcrk
  • dCRK
  • DCrk
  • Dmel\\CG1587
  • fb34h10
  • fc05a01
  • fi19g05
  • p38
  • P38C-CRK
  • v-crk
  • wu:fb34h10
  • wu:fc05a01
  • wu:fc54a04
  • wu:fi19g05
  • zgc:100936
Reactivity
Human
129
60
55
3
2
2
2
2
2
1
Host
Mouse
97
32
Clonality
Monoclonal
Conjugate
This Crk antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
78
44
25
23
22
22
16
15
11
4
4
2
2
Supplier
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Catalog No. ABIN969063
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2290752 ABIN969062 ICC FACS IHC ELISA WB Mouse IgG2b Log in to see 3G11C1 2
0.2290752 ABIN1532240 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.2290752 ABIN2705945 IHC WB Rabbit Center Log in to see Polyclonal
0.2290752 ABIN2163970 IF IHC ELISA WB Rabbit IgG Tyr221 Log in to see Polyclonal
0.2290752 ABIN2429829 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.2290752 ABIN2423205 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.2290752 ABIN1845971 ICC FACS IF IHC ELISA WB Mouse IgG2b Log in to see
0.2290752 ABIN1845972 ICC FACS IF IHC ELISA WB Mouse IgG2b Log in to see
0.2290752 ABIN3015531 IHC WB Rabbit Log in to see Polyclonal
0.0011325823 ABIN1497633 ICC FACS IF IHC ELISA WB Mouse IgG2b Log in to see 3G11E8
0.0011325823 ABIN1497632 ICC FACS IF IHC ELISA WB Mouse IgG2b Log in to see 3G11C1
0.0011325823 ABIN3184088 ELISA IF IHC WB Rabbit IgG Ser262 Log in to see Polyclonal
0.0011325823 ABIN2596632 IHC Rabbit Internal Region Log in to see Polyclonal
0.0011325823 ABIN2883099 IHC Rabbit IgG Log in to see Polyclonal
0.0011325823 ABIN1872005 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011325823 ABIN2138297 IF IHC ELISA WB Rabbit IgG pTyr221 Log in to see Polyclonal
0.0011325823 ABIN2241453 FACS IHC ELISA WB Mouse IgG2b Log in to see 13B190
0.0011325823 ABIN2241455 FACS IHC ELISA WB Mouse IgG2b Log in to see 13B191
0.0011325823 ABIN1843249 ICC IF IHC WB Rabbit Log in to see Polyclonal
0.0011325823 ABIN4300527 ELISA FACS ICC IF IHC WB Mouse IgG2b Log in to see 3G11E8

Top referenced anti-Crk antibody for Immunohistochemistry

Similar anti-Crk Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 22 Antibodies
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies
Reactivity Human
(129), (60), (55), (3), (2), (2), (2), (2), (2), (1)
Host Mouse
(97), (32)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Crk antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(78), (44), (25), (23), (22), (22), (16), (15), (11), (4), (4), (2), (2)
Pubmed 2 references available
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Product Details anti-Crk Antibody

Target Details Crk Application Details Handling References for anti-Crk Antibody (ABIN969063) Images
Purification Ascites
Immunogen Purified recombinant fragment of human CRK expressed in E. coli.
Clone 3G11E8
Isotype IgG2b

Target Details Crk

Product Details anti-Crk Antibody Application Details Handling References for anti-Crk Antibody (ABIN969063) Images back to top
Antigen
Alternative Name CRK (CRK Antibody Abstract)
Background This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Molecular Weight 42 kDa
Gene ID 1398
HGNC 1398
Pathways TCR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Crk Antibody Target Details Crk Handling References for anti-Crk Antibody (ABIN969063) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Crk Antibody Target Details Crk Application Details References for anti-Crk Antibody (ABIN969063) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Crk Antibody (ABIN969063)

Product Details anti-Crk Antibody Target Details Crk Application Details Handling Images back to top
Product cited in:

Watanabe, Tsuda, Tanaka, Ohba, Kawaguchi, Majima, Sawa, Minami: "Adaptor protein Crk induces Src-dependent activation of p38 MAPK in regulation of synovial sarcoma cell proliferation." in: Molecular cancer research : MCR, Vol. 7, Issue 9, pp. 1582-92, 2009

Tanaka: "[Biological roles of signaling adaptor protein CRK]." in: Seikagaku. The Journal of Japanese Biochemical Society, Vol. 81, Issue 5, pp. 361-76, 2009

Images

Product Details anti-Crk Antibody Target Details Crk Application Details Handling References for anti-Crk Antibody (ABIN969063) back to top
Supplier Images
ELISA image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 2: Flow cytometric analysis of Hela cells using CRK mouse mAb (blue) and negat...
Immunocytochemistry (ICC) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 3: Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 4: Immunohistochemical analysis of paraffin-embedded intima cancer tissues usi...
Immunohistochemistry (IHC) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using...
Western Blotting (WB) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 6: Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombina...
Western Blotting (WB) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (ABIN969063) Figure 7: Western blot analysis using CRK mAb against HEK293 (1) and CRK (AA: 1-204) ...