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|Antigen||V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Crk antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-Crk AntibodyTarget Details Crk Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-Crk2 protein only when phosphorylated at Try221.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details CrkProduct Details anti-Crk Antibody Application Details Handling Images back to top
|Alternative Name||CRK (CRK Antibody Abstract)|
|Background||The Crk family of adapter proteins including Crk II, Crk I and Crk L consist mostly of SH2 and SH3 domains. Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline- rich motifs, they are involved in a variety of signaling cascades. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor-I (IGFI) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38|
|Molecular Weight||approx. 42 kDa|
|Pathways||Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-Crk Antibody Target Details Crk Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Crk Antibody Target Details Crk Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Crk Antibody Target Details Crk Application Details Handling back to top