|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|BioImaging (BI)||2 Antibodies||2 Antibodies|
|ELISA||13 Antibodies||15 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||6 Antibodies|
|Immunofluorescence (IF)||11 Antibodies||14 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||38 Antibodies|
|Antigen||V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Crk antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Crk AntibodyTarget Details Crk Application Details Handling Images
|Specificity||This antibody detects endogenous levels of CRK protein (region surrounding aa215).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details CrkProduct Details anti-Crk Antibody Application Details Handling Images back to top
|Alternative Name||CRK (CRK Antibody Abstract)|
|Background||Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38|
|Molecular Weight||approx. 42 kDa|
|Pathways||TCR Signaling, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-Crk Antibody Target Details Crk Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Crk Antibody Target Details Crk Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
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