You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) Crk antibody for Immunoprecipitation

Recommended Crk Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies
  • c-Crk
  • CG1587
  • crk
  • CRK
  • Crk-I
  • Crk-II
  • Crk-III
  • crk2
  • Crk3
  • crkII
  • CRKII
  • CrkIII
  • Crko
  • D-CRK
  • D-Crk
  • dCrk
  • Dcrk
  • dCRK
  • DCrk
  • Dmel\\CG1587
  • fb34h10
  • fc05a01
  • fi19g05
  • p38
  • P38C-CRK
  • v-crk
  • wu:fb34h10
  • wu:fc05a01
  • wu:fc54a04
  • wu:fi19g05
  • zgc:100936
Epitope
AA 102-304
23
16
12
6
5
4
3
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
130
60
54
2
2
2
1
1
1
1
Host
Mouse
98
32
Clonality
Monoclonal
Conjugate
This Crk antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
Application
BioImaging (BI), Immunoprecipitation (IP), Western Blotting (WB)
79
45
26
24
22
22
18
15
12
4
3
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967703
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN967702 BI IP WB Mouse IgG2a AA 102-304 Log in to see 22-Crk 7
0.00089556724 ABIN2736584 ICC FACS IP WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN3059629 ICC FACS IF IP WB Rabbit Log in to see Polyclonal

Top referenced anti-Crk antibody for Immunoprecipitation

Similar anti-Crk Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Frog
Western Blotting (WB) 81 Antibodies 36 Antibodies 30 Antibodies 2 Antibodies 2 Antibodies 3 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 5 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 4 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 12 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 24 Antibodies 10 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies
Epitope AA 102-304
(23), (16), (12), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(130), (60), (54), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(98), (32)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Crk antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application BioImaging (BI), Immunoprecipitation (IP), Western Blotting (WB)
(79), (45), (26), (24), (22), (22), (18), (15), (12), (4), (3), (2), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-Crk Antibody

Target Details Crk Application Details Handling References for anti-Crk Antibody (ABIN967703) Images
Cross-Reactivity Cow (Bovine), Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Crk aa. 102-304
Clone 22-Crk
Isotype IgG2a

Target Details Crk

Product Details anti-Crk Antibody Application Details Handling References for anti-Crk Antibody (ABIN967703) Images back to top
Antigen
Alternative Name Crk (CRK Antibody Abstract)
Background Crk was first isolated as the v-crk oncogene from chicken retroviruses CT10 and ASV-1. All human cell lines examined to date express a 40 kDa Crk protein. In addition, there is variable expression of 42 kDa and 28 kDa Crk proteins. The c-crk gene is one of a class of genes, such as Nck and GRB2/ASH, which encode proteins that consist mainly of SH2 and SH3 domains. These proteins function as adaptor molecules in tyrosine kinase signal transduction pathways. The SH2 domains interact with phosphotyrosine-containing peptides, while the SH3 domains can enhance this interaction and/or bind to other cellular components. Both the SH2 and SH3 domains of the human Crk protein are required for differentiation of PC12 cells. Thus, Crk has a role in an NGF-induced signaling pathway that involves activation of p21ras. Furthermore, three proteins of 118 kDa, 125 kDa, and 136 kDa which specifically bind to the Crk SH3 domain have been identified.
Molecular Weight 40 kDa
Pathways TCR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Crk Antibody Target Details Crk Handling References for anti-Crk Antibody (ABIN967703) Images back to top
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only

Handling

Product Details anti-Crk Antibody Target Details Crk Application Details References for anti-Crk Antibody (ABIN967703) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Crk Antibody (ABIN967703)

Product Details anti-Crk Antibody Target Details Crk Application Details Handling Images back to top
Product cited in:

Miller, Chen, Gharib, Wang, Thomas, Misek, Giordano, Yee, Orringer, Hanash, Beer: "Increased C-CRK proto-oncogene expression is associated with an aggressive phenotype in lung adenocarcinomas." in: Oncogene, Vol. 22, Issue 39, pp. 7950-7, 2003

Cho, Klemke: "Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold." in: The Journal of cell biology, Vol. 156, Issue 4, pp. 725-36, 2002

Liu, Kimura, Baumann, Saltiel: "APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes." in: Molecular and cellular biology, Vol. 22, Issue 11, pp. 3599-609, 2002

Smith, Richardson, Kopf, Yoshida, Hollingsworth, Kornbluth: "Apoptotic regulation by the Crk adapter protein mediated by interactions with Wee1 and Crm1/exportin." in: Molecular and cellular biology, Vol. 22, Issue 5, pp. 1412-23, 2002

Girardin, Yaniv: "A direct interaction between JNK1 and CrkII is critical for Rac1-induced JNK activation." in: The EMBO journal, Vol. 20, Issue 13, pp. 3437-46, 2001

Smith, Evans, Murakami, Moyer, Moseley, Vande Woude, Kornbluth: "Wee1-regulated apoptosis mediated by the crk adaptor protein in Xenopus egg extracts." in: The Journal of cell biology, Vol. 151, Issue 7, pp. 1391-400, 2001

Fournier, Lamorte, Maroun, Lupher, Band, Langdon, Park: "Cbl-transforming variants trigger a cascade of molecular alterations that lead to epithelial mesenchymal conversion." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3397-410, 2000

Images

Product Details anti-Crk Antibody Target Details Crk Application Details Handling References for anti-Crk Antibody (ABIN967703) back to top
Supplier Images
Western Blotting (WB) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (AA 102-304) (ABIN967703) Western blot analysis of Crk on a HeLa lysate. Lane 1: 1:5000, lane 2: 1:10000, lane ...
Immunofluorescence (IF) image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (AA 102-304) (ABIN967703) Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well...
 image for anti-Crk antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)) (AA 102-304) (ABIN967703) anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (AA 102-304) antibody (Image 3)