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anti-Human CrkL antibody for Enzyme Immunoassay

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • 1110025F07Rik
  • AA589403
  • AI325100
  • CRKL
  • Crkol
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
Epitope
C-Term
37
18
16
15
13
13
12
12
9
7
5
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
133
77
54
2
1
1
Host
Goat
105
25
22
1
Clonality
Polyclonal
Conjugate
This CrkL antibody is un-conjugated
7
7
7
6
5
5
3
3
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
123
69
28
21
17
15
8
3
3
2
2
2
2
1
Supplier
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Available images

Catalog No. ABIN374141
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088206085 ABIN782884 EIA WB Goat C-Term Log in to see Polyclonal
0.00088206085 ABIN1106836 EIA WB Mouse IgG1 Log in to see 5-00E-012

Similar anti-CrkL Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 12 Antibodies 28 Antibodies 56 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 4 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 17 Antibodies 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 12 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Western Blotting (WB) 31 Antibodies 52 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Epitope C-Term
(37), (18), (16), (15), (13), (13), (12), (12), (9), (7), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(133), (77), (54), (2), (1), (1)
Host Goat
(105), (25), (22), (1)
Clonality
Polyclonal
Conjugate This CrkL antibody is un-conjugated
(7), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(123), (69), (28), (21), (17), (15), (8), (3), (3), (2), (2), (2), (2), (1)
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Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling Images
Sequence C-KIFDPQNPDE NE
Specificity This antibody detects CRLK at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide from C Terminus of the protein sequence according to NP_005198.1 Genename: CRKL

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling Images back to top
Antigen
Alternative Name CRKL (CRKL Antibody Abstract)
Background Crkl is a 33 kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.Synonyms: Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
Gene ID 1399
NCBI Accession NP_005198
UniProt P46109
Pathways TCR Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling Images back to top
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.01-0.03 μg/mL. Approx 37 kDa band observed in K562 and Jurkat lysates(calculated MW of 33.7 kDa according to NP_005198).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling back to top
Supplier Images
 image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (C-Term) (ABIN374141) anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (C-Term) antibody
 image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (C-Term) (ABIN374141) anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (C-Term) antibody (Image 2)