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anti-Human CrkL antibody for Flow Cytometry

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • 1110025F07Rik
  • AA589403
  • AI325100
  • CRKL
  • Crkol
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
Reactivity
Human
136
79
56
2
1
1
Host
Mouse
106
26
23
1
Clonality
Monoclonal
Conjugate
This CrkL antibody is un-conjugated
7
7
7
6
5
5
3
3
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
126
69
29
21
17
17
8
3
3
3
2
2
2
1
Supplier
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Catalog No. ABIN969064
€ 409.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30344847 ABIN1846114 FACS ELISA WB Mouse IgG1 Log in to see
0.0010486664 ABIN1497634 FACS ELISA WB Mouse IgG1 Log in to see 5E12
0.0010486664 ABIN965932 FACS ELISA WB Mouse IgG1 Log in to see 5E12 2
0.0010486664 ABIN220059 FACS IHC (p) IP WB Rabbit IgG pTyr207 Log in to see Polyclonal
0.0010486664 ABIN865399 FACS ELISA WB Mouse IgG1 pTry207 Log in to see 5E12
0.0010486664 ABIN2241482 FACS ELISA WB Mouse IgG1 Log in to see 13B192
0.0010486664 ABIN4300531 ELISA FACS WB Mouse IgG1 Log in to see 5-00E12
0.0010486664 ABIN2241534 FACS IP IHC WB Rabbit IgG pTyr207 Log in to see Polyclonal

Top referenced anti-CrkL antibody for Flow Cytometry

Similar anti-CrkL Antibodies

Application / Reactivity Human
ELISA 57 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 110 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Reactivity Human
(136), (79), (56), (2), (1), (1)
Host Mouse
(106), (26), (23), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CrkL antibody is un-conjugated
(7), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(126), (69), (29), (21), (17), (17), (8), (3), (3), (3), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling References for anti-CrkL Antibody (ABIN969064) Images
Purification Ascites
Immunogen Purified recombinant fragment of human CRKL expressed in E. coli.
Clone 5-00E-12
Isotype IgG1

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling References for anti-CrkL Antibody (ABIN969064) Images back to top
Antigen
Alternative Name CRKL (CRKL Antibody Abstract)
Background This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Molecular Weight 39 kDa
Gene ID 1399
HGNC 1399
Pathways TCR Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling References for anti-CrkL Antibody (ABIN969064) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details References for anti-CrkL Antibody (ABIN969064) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CrkL Antibody (ABIN969064)

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling Images back to top
Product cited in:

Seo, Suenaga, Hatakeyama, Taiji, Imamoto: "Structural and functional basis of a role for CRKL in a fibroblast growth factor 8-induced feed-forward loop." in: Molecular and cellular biology, Vol. 29, Issue 11, pp. 3076-87, 2009

Images

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling References for anti-CrkL Antibody (ABIN969064) back to top
Supplier Images
ELISA image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (ABIN969064) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (ABIN969064) Figure 2: Flow cytometric analysis of NIH/3T3 cells using CRKL mouse mAb (blue) and n...
Western Blotting (WB) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (ABIN969064) Figure 3: Western blot analysis using CRKL mAb against human CRKL (AA: 100-303) recom...