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anti-Human CrkL antibody for Western Blotting

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • 1110025F07Rik
  • AA589403
  • AI325100
  • CRKL
  • Crkol
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
Reactivity
Human
133
78
55
2
1
1
Host
Mouse
105
24
23
1
Clonality
Monoclonal
Conjugate
This CrkL antibody is un-conjugated
7
7
7
6
5
5
3
3
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
123
68
28
21
17
16
7
3
3
3
2
2
2
1
Supplier
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Catalog No. ABIN969064
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12511806 ABIN967605 WB Mouse IgG2a, kappa pTyr207 Log in to see K30-391-11-30 3
0.12511806 ABIN1531223 IF IHC ELISA WB Rabbit IgG pTyr207 Log in to see Polyclonal
0.12511806 ABIN2779341 WB Rabbit Middle Region Log in to see Polyclonal
0.12511806 ABIN657468 WB Rabbit Ig AA 50-79, N-Term Log in to see Polyclonal
0.12511806 ABIN514606 IF PLA RNAi ELISA WB Mouse IgG2b kappa AA 204-303, partial Log in to see 4B5 2
0.12511806 ABIN1532241 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12511806 ABIN3043046 WB Rabbit IgG AA 286-303, C-Term Log in to see Polyclonal
0.12511806 ABIN393757 WB Mouse IgG2b kappa pTry207 Log in to see 4B5
0.12511806 ABIN514605 IF PLA WB Rabbit AA 1-303, full length Log in to see Polyclonal 1
0.12511806 ABIN498177 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.12511806 ABIN2163973 IF IHC ELISA WB Rabbit IgG Tyr207 Log in to see Polyclonal
0.12511806 ABIN2463653 ELISA WB Rabbit Log in to see Polyclonal
0.12511806 ABIN2465201 ELISA WB Goat C-Term Log in to see Polyclonal
0.12511806 ABIN2160326 WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.12511806 ABIN926865 WB Rabbit Middle Region, pTry207 Log in to see Polyclonal
0.12511806 ABIN514603 IF WB Mouse AA 1-303, full length Log in to see Polyclonal
0.12511806 ABIN1106836 EIA WB Mouse IgG1 Log in to see 5-00E-012
0.12511806 ABIN514604 IF WB Mouse AA 1-303, full length Log in to see Polyclonal
0.12511806 ABIN2434506 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12511806 ABIN2432887 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-CrkL antibody for Western Blotting

Similar anti-CrkL Antibodies

Application / Reactivity Human
ELISA 56 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Reactivity Human
(133), (78), (55), (2), (1), (1)
Host Mouse
(105), (24), (23), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CrkL antibody is un-conjugated
(7), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(123), (68), (28), (21), (17), (16), (7), (3), (3), (3), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling References for anti-CrkL Antibody (ABIN969064) Images
Purification Ascites
Immunogen Purified recombinant fragment of human CRKL expressed in E. coli.
Clone 5-00E-12
Isotype IgG1

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling References for anti-CrkL Antibody (ABIN969064) Images back to top
Antigen
Alternative Name CRKL (CRKL Antibody Abstract)
Background This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Molecular Weight 39 kDa
Gene ID 1399
HGNC 1399
Pathways TCR Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling References for anti-CrkL Antibody (ABIN969064) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details References for anti-CrkL Antibody (ABIN969064) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CrkL Antibody (ABIN969064)

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling Images back to top
Product cited in:

Seo, Suenaga, Hatakeyama, Taiji, Imamoto: "Structural and functional basis of a role for CRKL in a fibroblast growth factor 8-induced feed-forward loop." in: Molecular and cellular biology, Vol. 29, Issue 11, pp. 3076-87, 2009

Images

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling References for anti-CrkL Antibody (ABIN969064) back to top
Supplier Images
ELISA image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (ABIN969064) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (ABIN969064) Figure 2: Flow cytometric analysis of NIH/3T3 cells using CRKL mouse mAb (blue) and n...
Western Blotting (WB) image for anti-CrkL antibody (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like) (ABIN969064) Figure 3: Western blot analysis using CRKL mAb against human CRKL (AA: 100-303) recom...