anti-Rat (Rattus) CrkL antibody for Intracellular Staining

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • CRKL
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
  • 1110025F07Rik
  • AA589403
  • AI325100
  • Crkol
  • v-crk sarcoma virus CT10 oncogene homolog (avian)-like
  • v-crk avian sarcoma virus CT10 oncogene homolog-like
  • CRKL
  • crkl
  • LOC100342327
  • Crkl
Epitope
pTyr207
30
29
17
17
15
14
14
12
10
8
7
4
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
169
88
65
3
3
3
2
1
1
1
1
1
1
Host
Mouse
124
42
22
1
Clonality
Monoclonal
Conjugate
This CrkL antibody is conjugated to Alexa Fluor 488
9
8
8
7
6
5
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
152
74
30
25
24
22
17
5
4
4
2
2
2
2
1
Options
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Catalog No. ABIN1177228
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177226 ICS PE Mouse IgG2a, kappa pTyr207 Log in to see K30-391-50-80 3
1 ABIN1177227 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr207 Log in to see K30-391-50-80 3

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Epitope pTyr207
(30), (29), (17), (17), (15), (14), (14), (12), (10), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(169), (88), (65), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(124), (42), (22), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CrkL antibody is conjugated to Alexa Fluor 488
(9), (8), (8), (7), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(152), (74), (30), (25), (24), (22), (17), (5), (4), (4), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling References for anti-CrkL antibody (ABIN1177228) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human, Mouse, and Rat CrkL Peptide
Clone K30-391-50-80
Isotype IgG2a, kappa

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling References for anti-CrkL antibody (ABIN1177228) Images back to top
Antigen
Alternative Name CrkL (CRKL Antibody Abstract)
Background Crk-Like (CrkL) is an adaptor protein that is preferentially expressed in hematopoietic cells and is encoded by a gene that is homologous to the viral oncogene v-crk (chicken tumor virus no. 10 regulator of kinase). Its SH2 and SH3 domains bind to a variety of effector proteins, such as paxillin, p130Cas, c-Cbl, c-Abl, and C3G. These interactions are involved in the regulation of cellular migration, adhesion, and transformation. Tyrosine 207 (Y207) of CrkL is phosphorylated in hematopoietic cells that express the BCR-ABL fusion protein. This site may be a negative regulator of protein complex formation and biological activity. The K30-391.50.80 monoclonal antibody recognizes the phosphorylated Y207 of human CrkL.
Research Area Transcription Factors
Pathways Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling References for anti-CrkL antibody (ABIN1177228) Images back to top
Application Notes This Alexa Fluor® 488-conjugated antibody is suitable for intracellular staining of cell lines using BD Cytofix™ Fixation Buffer and BD Phosflow Perm Buffer III or IV. We have confirmed that the Alexa Fluor® 647 conjugate, Cat. No. 560790, is suitable for staining leukocytes from human peripheral blood and bone marrow.
Comment

BALB/c

Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details References for anti-CrkL antibody (ABIN1177228) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CrkL antibody (ABIN1177228)

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling Images back to top
Product cited in:

Feller: "Crk family adaptors-signalling complex formation and biological roles." in: Oncogene, Vol. 20, Issue 44, pp. 6348-71, 2001

Arai, Nosaka, Kohsaka, Miyasaka, Miura: "CrkL activates integrin-mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G." in: Blood, Vol. 93, Issue 11, pp. 3713-22, 1999

Senechal, Heaney, Druker, Sawyers: "Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells." in: Molecular and cellular biology, Vol. 18, Issue 9, pp. 5082-90, 1998