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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||107 Antibodies||52 Antibodies||31 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||17 Antibodies||12 Antibodies||12 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies||19 Antibodies||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||21 Antibodies||21 Antibodies||21 Antibodies|
|Antigen||V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CrkL antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-CrkL AntibodyTarget Details CrkL Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-CrkL protein only when phosphorylated at Try207.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details CrkLProduct Details anti-CrkL Antibody Application Details Handling Images back to top
|Alternative Name||CRKL (CRKL Antibody Abstract)|
|Background||Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.Synonyms: Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like|
|Molecular Weight||approx. 39 kDa|
|Pathways||TCR Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CrkL Antibody Target Details CrkL Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CrkL Antibody Target Details CrkL Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CrkL Antibody Target Details CrkL Application Details Handling back to top