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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Cow (Bovine)||Dog (Canine)||Frog|
|Western Blotting (WB)||91 Antibodies||19 Antibodies||17 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies||3 Antibodies||4 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies||3 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies||14 Antibodies|
|Antigen||NCK Adaptor Protein 1 (NCK1) Antibodies|
|Epitope||AA 279-377 Alternatives|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cytoplasmic Protein NCK1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
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Product Details anti-Cytoplasmic Protein NCK1 AntibodyTarget Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN967743) Images
|Cross-Reactivity||Cow (Bovine), Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human NCK aa. 279-377|
Target Details Cytoplasmic Protein NCK1Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN967743) Images back to top
|Alternative Name||NCK (NCK1 Antibody Abstract)|
|Background||NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||47 kDa|
Application DetailsProduct Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN967743) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN967743) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN967743)Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
|Product cited in:||
Miyoshi-Akiyama, Aleman, Smith et al.: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." in: Oncogene, Vol. 20, Issue 30, pp. 4058-69, 2001 (PubMed).
Schmitz, Thömmes, Beier et al.: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, 2001 (PubMed).
Dalva, Takasu, Lin et al.: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." in: Cell, Vol. 103, Issue 6, pp. 945-56, 2001 (PubMed).
McManus, Boerner, Danielsen et al.: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, 2000 (PubMed).
Roche, McGlade, Jones et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." in: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, 1996 (PubMed).
ImagesProduct Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN967743) back to top