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anti-Human Cytoplasmic Protein NCK1 antibody for Flow Cytometry

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • NCK
  • Nck-1
  • nck-1
  • NCK1
  • NCKalpha
Epitope
AA 3-29, N-Term
35
15
5
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
95
19
17
3
2
2
2
2
Host
Rabbit
70
25
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
4
4
4
4
4
4
Application
Flow Cytometry (FACS), Western Blotting (WB)
90
52
41
13
10
7
7
3
2
1
1
Supplier
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Catalog No. ABIN652772
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
0.3068567 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
0.3068567 ABIN2446710 FACS ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.3068567 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0010666476 ABIN1499651 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7
0.0010666476 ABIN189565 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see Y531
0.0010666476 ABIN1811812 FACS WB Rabbit AA 9-38 Log in to see Polyclonal
0.0010666476 ABIN2318041 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B556

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Flow Cytometry

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 91 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 3-29, N-Term
(35), (15), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(95), (19), (17), (3), (2), (2), (2), (2)
Host Rabbit
(70), (25), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Flow Cytometry (FACS), Western Blotting (WB)
(90), (52), (41), (13), (10), (7), (7), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human NCK1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-29 AA from the N-terminal region of human NCK1.
Clone RB23609
Isotype Ig

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.
Synonyms: SH2/SH3 adaptor protein NCK-alpha, NCK adaptor protein 1, Nck-1, Cytoplasmic protein NCK1, NCK1, NCK
Molecular Weight 42864 DA
Gene ID 4690
UniProt P16333
Research Area Translation Factors, Signaling, Protein Modifications, Neurology
Pathways TCR Signaling

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images back to top
Format Liquid
Concentration 0.4 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Meisenhelder, Hunter: "The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5843-56, 1992

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 3-29) (ABIN652772) Western blot analysis of NCK1 Antibody (N-term) (ABIN652772) in Hela cell line lysate...
Flow Cytometry (FACS) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 3-29) (ABIN652772) NCK1 Antibody (N-term) (ABIN652772) flow cytometry analysis of MDA-MB231 cells (botto...