anti-Human Cytoplasmic Protein NCK1 antibody for Flow Cytometry

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK1
  • NCK
  • NCKalpha
  • nck-1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK1
  • LOC100347965
  • LOC100358703
  • Nck1
Epitope
AA 3-29, N-Term
28
26
14
5
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
146
32
26
18
8
5
5
3
3
2
2
2
2
2
2
2
1
1
Host
Rabbit
75
70
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
6
6
6
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
139
89
62
25
15
14
8
5
4
4
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN652772
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.326361 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
14.326361 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
14.326361 ABIN4338263 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7
14.326361 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN1499651 ELISA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7
1 ABIN4880888 ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7
1 ABIN5626304 ELISA FACS IHC WB DyLight 650 Mouse IgG1 Log in to see 5B7
1 ABIN5626302 ELISA FACS IHC WB DyLight 488 Mouse IgG1 Log in to see 5B7
1 ABIN5626307 ELISA FACS IHC FITC Mouse IgG1 Log in to see 5B7
1 ABIN5626295 ELISA FACS IHC WB Alexa Fluor 488 Mouse IgG1 Log in to see 5B7
1 ABIN5626297 ELISA FACS IHC WB Alexa Fluor 700 Mouse IgG1 Log in to see 5B7
1 ABIN5626309 FACS PerCP Mouse IgG1 Log in to see 5B7
1 ABIN5626294 ELISA FACS IHC WB Alexa Fluor 405 Mouse IgG1 Log in to see 5B7
1 ABIN5626296 ELISA FACS IHC WB Alexa Fluor 647 Mouse IgG1 Log in to see 5B7
1 ABIN5626300 ELISA FACS IHC WB DyLight 350 Mouse IgG1 Log in to see 5B7
1 ABIN5626301 ELISA FACS IHC WB DyLight 405 Mouse IgG1 Log in to see 5B7
1 ABIN5626303 ELISA FACS IHC WB DyLight 550 Mouse IgG1 Log in to see 5B7
1 ABIN5626305 ELISA FACS IHC WB DyLight 680 Mouse IgG1 Log in to see 5B7
1 ABIN5626306 ELISA FACS IHC WB DyLight 755 Mouse IgG1 Log in to see 5B7
1 ABIN5626298 FACS Allophycocyanin Mouse IgG1 Log in to see 5B7

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 3-29, N-Term
(28), (26), (14), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(146), (32), (26), (18), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(75), (70), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(6), (6), (6), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(139), (89), (62), (25), (15), (14), (8), (5), (4), (4), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human NCK1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-29 AA from the N-terminal region of human NCK1.
Clone RB23609
Isotype Ig

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.
Synonyms: SH2/SH3 adaptor protein NCK-alpha, NCK adaptor protein 1, Nck-1, Cytoplasmic protein NCK1, NCK1, NCK
Molecular Weight 42864 DA
Gene ID 4690
UniProt P16333
Research Area Translation Factors, Signaling, Protein Modifications, Neurology
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images back to top
Format Liquid
Concentration 0.4 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Meisenhelder, Hunter: "The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5843-56, 1992

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) back to top
Supplier Images
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 3-29), (N-Term) antibody (ABIN652772) Western blot analysis of NCK1 Antibody (N-term) (ABIN652772) in Hela cell line lysate...
Flow Cytometry (FACS) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 3-29), (N-Term) antibody (ABIN652772) NCK1 Antibody (N-term) (ABIN652772) flow cytometry analysis of MDA-MB231 cells (botto...