anti-Human Cytoplasmic Protein NCK1 antibody for Immunocytochemistry

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK1
  • NCK
  • NCKalpha
  • nck-1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK1
  • LOC100347965
  • LOC100358703
  • Nck1
Epitope
AA 53-333
29
26
14
5
4
3
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
128
31
26
8
5
5
3
3
2
2
2
2
2
2
2
1
1
1
Host
Rabbit
74
53
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
125
75
48
14
13
11
8
4
4
4
1
1
1
Supplier
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Catalog No. ABIN1499652
$ 507.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4338265 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN5078297 ICC IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN3200710 ICC WB Mouse IgG1 Log in to see EM-06
1 ABIN5014021 ICC IHC IP WB Rabbit IgG AA 2-377 Log in to see Polyclonal

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 75 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 126 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 53-333
(29), (26), (14), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(128), (31), (26), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(74), (53), (1)
Clonality
Polyclonal
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(125), (75), (48), (14), (13), (11), (8), (4), (4), (4), (1), (1), (1)
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling Images
Specificity Human NCK1
Predicted Reactivity Percent identity by BLAST analysis: Human (100%) Rat, Bovine (99%) Mouse (98%) Porcine (97%) Xenopus (84%).
Purification Immunoaffinity purified
Immunogen Recombinant fragment corresponding to a region within amino acids 53 and 333 of NCK1 (SwissProt P16333). Percent identity by BLAST analysis: Human (100%), Rat, Bovine (99%), Mouse (98%), Porcine (97%), Xenopus (84%).

Type of Immunogen: Recombinant protein
Isotype IgG

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NCK1 / NCK (NCK1 Antibody Abstract)
Background Name/Gene ID: NCK1

Synonyms: NCK1, Cytoplasmic protein nck, Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, NCKalpha, Melanoma NCK protein, NCK, NCK tyrosine kinase
Gene ID 4690
UniProt P16333
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling Images back to top
Application Notes Approved: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling back to top
Supplier Images
 image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 53-333) (ABIN1499652) anti-NCK Adaptor Protein 1 (NCK1) (AA 53-333) antibody
 image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 53-333) (ABIN1499652) anti-NCK Adaptor Protein 1 (NCK1) (AA 53-333) antibody (Image 2)
 image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 53-333) (ABIN1499652) anti-NCK Adaptor Protein 1 (NCK1) (AA 53-333) antibody (Image 3)