anti-Human Cytoplasmic Protein NCK1 antibody for Western Blotting

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK1
  • NCK
  • NCKalpha
  • nck-1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK1
  • LOC100347965
  • LOC100358703
  • Nck1
Epitope
AA 3-29, N-Term
28
26
14
5
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
128
31
26
8
5
5
3
3
2
2
2
2
2
2
2
1
1
1
Host
Rabbit
74
53
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
125
75
48
15
14
10
8
5
4
4
1
1
1
Options
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Catalog No. ABIN652772
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3484275 ABIN657648 IHC (p) WB Rabbit Ig AA 63-92, N-Term Log in to see Polyclonal 3
3.3484275 ABIN453756 EIA WB Rabbit N-Term Log in to see Polyclonal 1
3.3484275 ABIN393431 WB Mouse IgG Mix kappa AA 185-294 Log in to see 1A1 5
3.3484275 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
3.3484275 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
3.3484275 ABIN967744 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
3.3484275 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
3.3484275 ABIN2789859 WB Rabbit N-Term Log in to see Polyclonal
3.3484275 ABIN3197734 IC IF WB Rabbit Log in to see Polyclonal
3.3484275 ABIN4338265 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.3484275 ABIN4338266 ELISA WB Mouse IgG3 kappa AA 1-377 Log in to see 6B3
3.3484275 ABIN4338263 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7
3.3484275 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3484275 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal
3.3484275 ABIN4338264 ELISA IHC (p) WB Mouse IgG1 kappa Log in to see 11D10
3.3484275 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal
3.3484275 ABIN5078297 ICC IF WB Rabbit IgG Log in to see Polyclonal
3.3484275 ABIN518212 IF WB Mouse AA 1-377, full length Log in to see Polyclonal
3.3484275 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294, partial Log in to see 1A1
3.3484275 ABIN1327536 IF WB Mouse AA 1-377, full length Log in to see Polyclonal

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Western Blotting

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 75 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 126 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 3-29, N-Term
(28), (26), (14), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(128), (31), (26), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(74), (53), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(125), (75), (48), (15), (14), (10), (8), (5), (4), (4), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling ProductDetails: References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human NCK1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-29 AA from the N-terminal region of human NCK1.
Clone RB23609
Isotype Ig

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling ProductDetails: References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.
Synonyms: SH2/SH3 adaptor protein NCK-alpha, NCK adaptor protein 1, Nck-1, Cytoplasmic protein NCK1, NCK1, NCK
Molecular Weight 42864 DA
Gene ID 4690
UniProt P16333
Research Area Translation Factors, Signaling, Protein Modifications, Neurology
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling ProductDetails: References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details ProductDetails: References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) Images back to top
Format Liquid
Concentration 0.4 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Meisenhelder, Hunter: "The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5843-56, 1992

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling ProductDetails: References for anti-Cytoplasmic Protein NCK1 antibody (ABIN652772) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 3-29) (ABIN652772) Western blot analysis of NCK1 Antibody (N-term) (ABIN652772) in Hela cell line lysate...
Flow Cytometry (FACS) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 3-29) (ABIN652772) NCK1 Antibody (N-term) (ABIN652772) flow cytometry analysis of MDA-MB231 cells (botto...