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anti-Human Cytoplasmic Protein NCK1 antibody for Western Blotting

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • NCK
  • Nck-1
  • nck-1
  • NCK1
  • NCKalpha
Epitope
AA 3-29, N-Term
35
24
15
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
119
21
18
4
2
2
2
2
1
Host
Rabbit
72
47
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
114
74
44
14
12
9
7
4
2
1
1
Supplier
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Catalog No. ABIN652772
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN657648 IHC (p) WB Rabbit Ig AA 63-92, N-Term Log in to see Polyclonal 3
0.124960825 ABIN453756 EIA WB Rabbit N-Term Log in to see Polyclonal 1
0.124960825 ABIN393431 WB Mouse IgG Mix kappa AA 185-294 Log in to see 1A1 5
0.124960825 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
0.124960825 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
0.124960825 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
0.124960825 ABIN967744 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
0.124960825 ABIN2789859 WB Rabbit N-Term Log in to see Polyclonal
0.124960825 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN518212 IF WB Mouse AA 1-377, full length Log in to see Polyclonal
0.124960825 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294, partial Log in to see 1A1
0.124960825 ABIN2446711 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.124960825 ABIN3197734 IC IF WB Rabbit Log in to see Polyclonal
0.124960825 ABIN518213 PLA WB Rabbit AA 1-377, full length Log in to see Polyclonal
0.124960825 ABIN1327536 IF WB Mouse AA 1-377, full length Log in to see Polyclonal
0.124960825 ABIN2421903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN2428459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN2446710 FACS ELISA WB Rabbit Ig Fraction Log in to see Polyclonal

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Western Blotting

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 114 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 3-29, N-Term
(35), (24), (15), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(119), (21), (18), (4), (2), (2), (2), (2), (1)
Host Rabbit
(72), (47), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(114), (74), (44), (14), (12), (9), (7), (4), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human NCK1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-29 AA from the N-terminal region of human NCK1.
Clone RB23609
Isotype Ig

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.
Synonyms: SH2/SH3 adaptor protein NCK-alpha, NCK adaptor protein 1, Nck-1, Cytoplasmic protein NCK1, NCK1, NCK
Molecular Weight 42864 DA
Gene ID 4690
UniProt P16333
Research Area Translation Factors, Signaling, Protein Modifications, Neurology
Pathways TCR Signaling

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) Images back to top
Format Liquid
Concentration 0.4 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Meisenhelder, Hunter: "The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5843-56, 1992

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN652772) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 3-29) (ABIN652772) Western blot analysis of NCK1 Antibody (N-term) (ABIN652772) in Hela cell line lysate...
Flow Cytometry (FACS) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 3-29) (ABIN652772) NCK1 Antibody (N-term) (ABIN652772) flow cytometry analysis of MDA-MB231 cells (botto...