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anti-Human Cytoplasmic Protein NCK1 antibody for Western Blotting

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK1
  • NCK
  • NCKalpha
  • nck-1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK1
  • LOC100347965
  • LOC100358703
  • Nck1
Epitope
AA 63-92, N-Term
28
24
14
5
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
122
30
25
8
4
4
2
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
73
48
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
119
75
47
15
12
10
7
4
3
2
1
1
1
Options
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Catalog No. ABIN657648
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1002488 ABIN652772 FACS WB Rabbit Ig AA 3-29, N-Term Log in to see Polyclonal 1
3.1002488 ABIN453756 EIA WB Rabbit N-Term Log in to see Polyclonal 1
3.1002488 ABIN393431 WB Mouse IgG Mix kappa AA 185-294 Log in to see 1A1 5
3.1002488 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
3.1002488 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
3.1002488 ABIN967744 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
3.1002488 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
3.1002488 ABIN2789859 WB Rabbit N-Term Log in to see Polyclonal
3.1002488 ABIN3197734 IC IF WB Rabbit Log in to see Polyclonal
3.1002488 ABIN4338265 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.1002488 ABIN4338266 ELISA WB Mouse IgG3 kappa AA 1-377 Log in to see 6B3
3.1002488 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1002488 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal
3.1002488 ABIN4338263 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7
3.1002488 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal
3.1002488 ABIN4338264 ELISA IHC (p) WB Mouse IgG1 kappa Log in to see 11D10
3.1002488 ABIN518212 IF WB Mouse AA 1-377, full length Log in to see Polyclonal
3.1002488 ABIN2446711 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
3.1002488 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294, partial Log in to see 1A1
3.1002488 ABIN2421903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Western Blotting

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 75 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 120 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 63-92, N-Term
(28), (24), (14), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(122), (30), (25), (8), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(73), (48), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(119), (75), (47), (15), (12), (10), (7), (4), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 AA from the N-terminal region of human NCK1.
Clone RB33697
Isotype Ig

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Synonyms: Cytoplasmic protein NCK1,NCK1,NCK
Molecular Weight 42864 DA
Gene ID 4690
NCBI Accession NP_006144, NP_001177725
UniProt P16333
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways TCR Signaling, Regulation of Actin Filament Polymerization

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment NCK1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months

References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Barda-Saad, Shirasu, Pauker, Hassan, Perl, Balbo, Yamaguchi, Houtman, Appella, Schuck, Samelson: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010

Gehmlich, Hayess, Legler, Haebel, Van der Ven, Ehler, Fürst: "Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells." in: European journal of cell biology, Vol. 89, Issue 5, pp. 351-64, 2010

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 63-92) (ABIN657648) Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) ...