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anti-Dog (Canine) DBNL antibody for Western Blotting

Recommended DBNL Antibody (supplied by: Log in to see )

Antigen
Drebrin-Like (DBNL) Antibodies
  • ABP1
  • Abp1
  • abp1
  • dbnl
  • DBNL
  • dbnl-B
  • DKFZp459C0939
  • HIP-55
  • HIP55
  • mAbp1
  • SH3P7
  • Sh3p7
  • wu:fb34f02
  • zgc:91835
  • zgc:112450
Epitope
N-Term
17
15
13
2
2
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
53
11
8
1
1
1
1
1
1
Host
Goat
34
14
5
Clonality
Polyclonal
Conjugate
This DBNL antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
45
30
8
8
2
2
2
Supplier
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Catalog No. ABIN374214
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011794439 ABIN2563044 ELISA WB Goat IgG N-Term Log in to see Polyclonal

Top referenced anti-DBNL antibody for Western Blotting

Similar anti-DBNL Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Rat (Rattus)
Western Blotting (WB) 48 Antibodies 10 Antibodies 2 Antibodies 7 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 9 Antibodies 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Drebrin-Like (DBNL) Antibodies
Epitope N-Term
(17), (15), (13), (2), (2), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(53), (11), (8), (1), (1), (1), (1), (1), (1)
Host Goat
(34), (14), (5)
Clonality
Polyclonal
Conjugate This DBNL antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(45), (30), (8), (8), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-DBNL Antibody

Target Details DBNL Application Details Handling References for anti-DBNL Antibody (ABIN374214) Images
Sequence AANLSRNGPA LQE-C
Specificity This antibody is expected to recognise all reported isoforms (NP_054782.2, NP_001014436.1, NP_001116428.1).
Cross-Reactivity (Details) Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide from N Terminus of the protein sequence according to NP_054782.2, NP_001014436.1, NP_001116428.1. Genename: DBNL

Target Details DBNL

Product Details anti-DBNL Antibody Application Details Handling References for anti-DBNL Antibody (ABIN374214) Images back to top
Antigen
Alternative Name Drebrin-Like Protein (DBNL) (DBNL Antibody Abstract)
Background Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. Several actin binding proteins, WASP, cortactin, and Abp1, regulate the activity of the Arp2/3 complex. HIP55 is the mammalian homologue of the yeast HIP55, which has been shown to bind actin and to have roles in endocytosis. HIP55 contains a C-terminal Src homology 3 domain and an N-terminal actin depolymerization factor (ADF-H/C) domain that is homologous to a similar domain found in the actin severing protein, cofilin. HIP55 can bind Src kinases, hematopoietic progenitor kinase 1 (HPK-1), myosin I heavy chain kinase, and dynamin. Thus, HIP55 may be important for regulation of the actin cytoskeleton through its ability to interact with F-actin and various other cytoskeletal and cell signaling proteins. There are three different isoforms.Synonyms: CMAP, Cervical mucin-associated protein, Drebrin-F, HIP-55, HPK1-interacting protein of 55 kDa, SH3 domain-containing protein 7, SH3P7
Molecular Weight 49kDa (NP_054782).
Gene ID 28988
NCBI Accession NP_054782
UniProt Q9UJU6
Pathways TCR Signaling

Application Details

Product Details anti-DBNL Antibody Target Details DBNL Handling References for anti-DBNL Antibody (ABIN374214) Images back to top
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 55 kDa band observed in Jurkat and 3T3 lysates (predictedMW of 45 kDa according to NP_054782).
Restrictions For Research Use only

Handling

Product Details anti-DBNL Antibody Target Details DBNL Application Details References for anti-DBNL Antibody (ABIN374214) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DBNL Antibody (ABIN374214)

Product Details anti-DBNL Antibody Target Details DBNL Application Details Handling Images back to top
Background publications

Ensenat, Yao, Wang, Kori, Zhou, Lee, Tan: "A novel src homology 3 domain-containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1." in: The Journal of biological chemistry, Vol. 274, Issue 48, pp. 33945-50, 1999

Images

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Supplier Images
 image for anti-DBNL antibody (Drebrin-Like) (N-Term) (ABIN374214) anti-Drebrin-Like (DBNL) (N-Term) antibody