anti-Human FYB antibody for ELISA

Recommended FYB Antibody (supplied by: Log in to see )

Antigen
FYN-Binding Protein (FYB) Antibodies
  • fyb
  • ADAP
  • PRO0823
  • SLAP-130
  • B630013F22Rik
  • FYB-120/130
  • Slap130
  • p120/130
  • p120/p130
  • RGD1563421
  • FYN-binding protein
  • FYN binding protein
  • fyb
  • FYB
  • Fyb
Epitope
N-Term
21
12
9
9
6
2
2
2
1
1
1
Reactivity
Human
98
27
21
6
5
4
3
3
2
2
Host
Rabbit
58
30
9
2
Clonality
Polyclonal
Conjugate
This FYB antibody is un-conjugated
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
63
38
18
13
11
6
4
4
4
2
2
Supplier
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Catalog No. ABIN1001739
$ 466.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1001738 ICC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 4
1 ABIN3184706 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN203719 ICC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1589991 ELISA WB Goat Log in to see Polyclonal
1 ABIN1833959 ELISA WB Goat AA 70-80 Log in to see Polyclonal
1 ABIN1030221 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1031214 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2614517 ELISA WB Rabbit IgG AA 645-675 Log in to see Polyclonal
1 ABIN2270425 ELISA WB Rabbit IgG C-Term, AA 645-675 Log in to see Polyclonal
1 ABIN2562707 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1891447 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 646-675 Log in to see Polyclonal
1 ABIN1891449 ELISA WB Biotin Rabbit IgG AA 646-675 Log in to see Polyclonal
1 ABIN1891450 ELISA WB FITC Rabbit IgG AA 646-675 Log in to see Polyclonal
1 ABIN1891446 ELISA WB HRP Rabbit IgG AA 646-675 Log in to see Polyclonal
1 ABIN1891448 ELISA WB APC Rabbit IgG AA 646-675 Log in to see Polyclonal
1 ABIN1891451 ELISA WB PE Rabbit IgG AA 646-675 Log in to see Polyclonal
1 ABIN4901567 ELISA WB Mouse IgG1 Log in to see 2H9-H6-G10
1 ABIN2270427 ELISA WB APC Rabbit IgG C-Term, AA 645-675 Log in to see Polyclonal
1 ABIN2270430 ELISA WB HRP Rabbit IgG C-Term, AA 645-675 Log in to see Polyclonal
1 ABIN2270429 ELISA WB FITC Rabbit IgG C-Term, AA 645-675 Log in to see Polyclonal

General

Antigen FYN-Binding Protein (FYB) Antibodies
Epitope N-Term
(21), (12), (9), (9), (6), (2), (2), (2), (1), (1), (1)
Reactivity Human
(98), (27), (21), (6), (5), (4), (3), (3), (2), (2)
Host Rabbit
(58), (30), (9), (2)
Clonality
Polyclonal
Conjugate This FYB antibody is un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(63), (38), (18), (13), (11), (6), (4), (4), (4), (2), (2)
Pubmed 4 references available
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Product Details anti-FYB Antibody

Target Details FYB Application Details Handling References for anti-FYB antibody (ABIN1001739) Images
Blocking Peptide Blocking peptide for this product available: ABIN1004072
Purification Affinity chromatography purified via peptide column.
Immunogen ADAP antibody was raised in rabbits against a 17 amino acid peptide from near the amino terminus of human ADAP.
Isotype IgG

Target Details FYB

Product Details anti-FYB Antibody Application Details Handling References for anti-FYB antibody (ABIN1001739) Images back to top
Antigen
Alternative Name ADAP (FYB Antibody Abstract)
Background The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Pathways TCR Signaling, Protein targeting to Nucleus

Application Details

Product Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB antibody (ABIN1001739) Images back to top
Application Notes ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB antibody (ABIN1001739) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-FYB antibody (ABIN1001739)

Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
Product cited in:

Medeiros, Burbach, Mueller, Srivastava, Moon, Highfill, Peterson, Shimizu: "Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1." in: Science (New York, N.Y.), Vol. 316, Issue 5825, pp. 754-8, 2007

Mueller, Thomas, Burbach, Peterson, Shimizu: "Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3559-69, 2007

Rawlings, Sommer, Moreno-García: "The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes." in: Nature reviews. Immunology, Vol. 6, Issue 11, pp. 799-812, 2006

Griffiths, Krawczyk, Kong, Raab, Hyduk, Bouchard, Chan, Kozieradzki, Oliveira-Dos-Santos, Wakeham, Ohashi, Cybulsky, Rudd, Penninger: "Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap." in: Science (New York, N.Y.), Vol. 293, Issue 5538, pp. 2260-3, 2001