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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies|
|Western Blotting (WB)||59 Antibodies|
|Antigen||FYN-Binding Protein (FYB) Antibodies|
|Conjugate||This FYB antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-FYB AntibodyTarget Details FYB Application Details Handling References for anti-FYB Antibody (ABIN1001739) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN1004072|
|Purification||Affinity chromatography purified via peptide column.|
|Immunogen||ADAP antibody was raised in rabbits against a 17 amino acid peptide from near the amino terminus of human ADAP.|
Target Details FYBProduct Details anti-FYB Antibody Application Details Handling References for anti-FYB Antibody (ABIN1001739) Images back to top
|Alternative Name||ADAP (FYB Antibody Abstract)|
|Background||The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.|
Application DetailsProduct Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB Antibody (ABIN1001739) Images back to top
|Application Notes||ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB Antibody (ABIN1001739) Images back to top
|Buffer||Antibody is supplied in PBS containing 0.02% sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.|
|Expiry Date||12 months|
References for anti-FYB Antibody (ABIN1001739)Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
|Product cited in:||
Mueller, Thomas, Burbach et al.: "Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3559-69, 2007 (PubMed).
Medeiros, Burbach, Mueller et al.: "Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1." in: Science (New York, N.Y.), Vol. 316, Issue 5825, pp. 754-8, 2007 (PubMed).
Rawlings, Sommer, Moreno-García: "The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes." in: Nature reviews. Immunology, Vol. 6, Issue 11, pp. 799-812, 2006 (PubMed).
Griffiths, Krawczyk, Kong et al.: "Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap." in: Science (New York, N.Y.), Vol. 293, Issue 5538, pp. 2260-3, 2001 (PubMed).