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anti-Mouse (Murine) FYB antibody for ELISA

Recommended FYB Antibody (supplied by: Log in to see )

Antigen
FYN-Binding Protein (FYB) Antibodies
  • ADAP
  • B630013F22Rik
  • fyb
  • FYB-120/130
  • p120/130
  • p120/p130
  • PRO0823
  • RGD1563421
  • SLAP-130
  • Slap130
Epitope
C-Term
29
16
9
3
2
2
2
2
Reactivity
Human, Mouse (Murine)
73
23
17
2
2
Host
Rabbit
58
11
7
Clonality
Polyclonal
Conjugate
This FYB antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
61
38
13
11
6
4
3
2
2
2
Supplier
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Catalog No. ABIN1001738
$ 455.07
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00121214 ABIN3184706 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00121214 ABIN2155079 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00121214 ABIN2439900 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00121214 ABIN1030221 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00121214 ABIN1887297 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00121214 ABIN1583547 ICC ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-FYB antibody for ELISA

Similar anti-FYB Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 14 Antibodies
Immunofluorescence (IF) 1 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 14 Antibodies 59 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen FYN-Binding Protein (FYB) Antibodies
Epitope C-Term
(29), (16), (9), (3), (2), (2), (2), (2)
Reactivity Human, Mouse (Murine)
(73), (23), (17), (2), (2)
Host Rabbit
(58), (11), (7)
Clonality
Polyclonal
Conjugate This FYB antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(61), (38), (13), (11), (6), (4), (3), (2), (2), (2)
Pubmed 4 references available
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Product Details anti-FYB Antibody

Target Details FYB Application Details Handling References for anti-FYB Antibody (ABIN1001738) Images
Blocking Peptide Blocking peptide for this product available: ABIN1003727
Purification Affinity chromatography purified via peptide column.
Immunogen ADAP antibody was raised in rabbits against a 20 amino acid peptide from near the carboxy terminus of human ADAP.
Isotype IgG

Target Details FYB

Product Details anti-FYB Antibody Application Details Handling References for anti-FYB Antibody (ABIN1001738) Images back to top
Antigen
Alternative Name ADAP (FYB Antibody Abstract)
Background The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Pathways TCR Signaling

Application Details

Product Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB Antibody (ABIN1001738) Images back to top
Application Notes ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB Antibody (ABIN1001738) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-FYB Antibody (ABIN1001738)

Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
Product cited in:

Medeiros, Burbach, Mueller, Srivastava, Moon, Highfill, Peterson, Shimizu: "Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1." in: Science (New York, N.Y.), Vol. 316, Issue 5825, pp. 754-8, 2007

Mueller, Thomas, Burbach, Peterson, Shimizu: "Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3559-69, 2007

Rawlings, Sommer, Moreno-García: "The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes." in: Nature reviews. Immunology, Vol. 6, Issue 11, pp. 799-812, 2006

Griffiths, Krawczyk, Kong, Raab, Hyduk, Bouchard, Chan, Kozieradzki, Oliveira-Dos-Santos, Wakeham, Ohashi, Cybulsky, Rudd, Penninger: "Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap." in: Science (New York, N.Y.), Vol. 293, Issue 5538, pp. 2260-3, 2001