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anti-Human GAB2 antibody for Western Blotting

Recommended GAB2 Antibody (supplied by: Log in to see )

Antigen
GRB2-Associated Binding Protein 2 (GAB2) Antibodies
  • AI463667
  • D130058I17Rik
  • GAB2
  • p97
Epitope
pSer623
24
22
22
16
15
13
13
13
13
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
165
120
117
3
1
1
1
1
1
1
Host
Rabbit
150
15
1
Clonality
Polyclonal
Conjugate
This GAB2 antibody is un-conjugated
6
6
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
108
64
55
31
20
3
1
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Catalog No. ABIN1532145
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1532144 IHC ELISA WB Rabbit IgG pTyr643 Log in to see Polyclonal 1
0.12303223 ABIN1533171 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1533138 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.12303223 ABIN1533172 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1881358 FACS WB Rabbit AA 3-30, N-Term Log in to see Polyclonal 2
0.12303223 ABIN2465739 ELISA WB Goat Internal Region Log in to see Polyclonal
0.12303223 ABIN2706197 WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN253292 ELISA WB Goat IgG C-Term, both Isoforms Log in to see Polyclonal
0.12303223 ABIN2439919 ELISA WB Rabbit IgG Ser623 Log in to see Polyclonal
0.12303223 ABIN2439926 ELISA WB Rabbit IgG pSer159 Log in to see Polyclonal
0.12303223 ABIN2439928 IHC ELISA WB Rabbit IgG pSer623 Log in to see Polyclonal
0.12303223 ABIN2439930 IHC ELISA WB Rabbit IgG pTyr643 Log in to see Polyclonal
0.12303223 ABIN2439921 ELISA WB Rabbit IgG Tyr643 Log in to see Polyclonal
0.12303223 ABIN2439927 IHC ELISA WB Rabbit IgG Ser159 Log in to see Polyclonal
0.12303223 ABIN2437220 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12303223 ABIN3184718 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12303223 ABIN523270 WB Rabbit AA 1-676, full length Log in to see Polyclonal
0.12303223 ABIN2423499 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2430143 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2566145 WB Mouse AA 1-676, full length Log in to see Polyclonal

Top referenced anti-GAB2 antibody for Western Blotting

Similar anti-GAB2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 42 Antibodies 42 Antibodies 66 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies 55 Antibodies 55 Antibodies
Immunohistochemistry (IHC) 25 Antibodies 27 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies 16 Antibodies
Western Blotting (WB) 67 Antibodies 69 Antibodies
Flow Cytometry (FACS) 3 Antibodies

General

Antigen GRB2-Associated Binding Protein 2 (GAB2) Antibodies
Epitope pSer623
(24), (22), (22), (16), (15), (13), (13), (13), (13), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(165), (120), (117), (3), (1), (1), (1), (1), (1), (1)
Host Rabbit
(150), (15), (1)
Clonality
Polyclonal
Conjugate This GAB2 antibody is un-conjugated
(6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(108), (64), (55), (31), (20), (3), (1)
Pubmed 2 references available
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Product Details anti-GAB2 Antibody

Target Details GAB2 Application Details Handling References for anti-GAB2 Antibody (ABIN1532145) Images
Specificity Gab2 (Phospho-Ser623) Antibody detects endogenous levels of Gab2 only when phosphorylated at Ser623.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Gab2 around the phosphorylation site of Ser623.
Isotype IgG

Target Details GAB2

Product Details anti-GAB2 Antibody Application Details Handling References for anti-GAB2 Antibody (ABIN1532145) Images back to top
Antigen
Alternative Name Gab2 (GAB2 Antibody Abstract)
Background Molecular weight of target protein: 74 kDa
Synonyms: GRB2-associated-binding protein 2, Growth factor receptor bound protein 2-associated protein 2, GRB2-associated binder 2, pp100, KIAA0571
Molecular Weight 74 kDa
Gene ID 9846
OMIM 606203
UniProt Q9UQC2
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-GAB2 Antibody Target Details GAB2 Handling References for anti-GAB2 Antibody (ABIN1532145) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.429434 (NCBI Gene Symbol: GAB2)

Restrictions For Research Use only

Handling

Product Details anti-GAB2 Antibody Target Details GAB2 Application Details References for anti-GAB2 Antibody (ABIN1532145) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-GAB2 Antibody (ABIN1532145)

Product Details anti-GAB2 Antibody Target Details GAB2 Application Details Handling Images back to top
Background publications

Taylor, Noguchi, Totoki, Toyoda, Kuroki, Dewar, Lloyd, Itoh, Takeda, Kim, She, Barlow, Bloom, Bruford, Chang, Cuomo, Eichler, FitzGerald, Jaffe, LaButti, Nicol, Park, Seaman, Sougnez, Yang, Zimmer et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification. ..." in: Nature, Vol. 440, Issue 7083, pp. 497-500, 2006

Nishida, Yoshida, Itoh, Fukada, Ohtani, Shirogane, Atsumi, Takahashi-Tezuka, Ishihara, Hibi, Hirano: "Gab-family adapter proteins act downstream of cytokine and growth factor receptors and T- and B-cell antigen receptors." in: Blood, Vol. 93, Issue 6, pp. 1809-16, 1999

Images

Product Details anti-GAB2 Antibody Target Details GAB2 Application Details Handling References for anti-GAB2 Antibody (ABIN1532145) back to top
Supplier Images
Western Blotting (WB) image for anti-GAB2 antibody (GRB2-Associated Binding Protein 2) (pSer623) (ABIN1532145) Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30', usi...
Immunohistochemistry (IHC) image for anti-GAB2 antibody (GRB2-Associated Binding Protein 2) (pSer623) (ABIN1532145) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Gab2...