|Antigen||GATA Binding Protein 3 (GATA3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GATA3 antibody is un-conjugated Alternatives|
BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
|8 references available|
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Product Details anti-GATA3 AntibodyTarget Details GATA3 Application Details Handling References for anti-GATA3 antibody (ABIN967621) Images
|Cross-Reactivity (Details)||Predicted: Rat|
1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Conserved peptide between the trans-activation and DNA-binding domains of human, mouse and rat GATA3|
Target Details GATA3Product Details anti-GATA3 Antibody Application Details Handling References for anti-GATA3 antibody (ABIN967621) Images back to top
|Alternative Name||GATA3 (GATA3 Antibody Abstract)|
GATA3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This ~50-kDa nuclear protein regulates the development and subsequent maintenance of multiple tissues. GATA3 is involved in the development of T lymphocytes (regulates T cell receptor subunit gene expression) and the differentiation of mature T cells to become Th2 cells. The expressed levels of normal or mutant GATA3 are also associated with the behaviors of various cancer cells including estrogen receptor-positive breast carcinoma cells.
The L50-823 monoclonal antibody recognizes human and mouse GATA3.
|Molecular Weight||50 kDa|
|Research Area||Lineage Markers, Transcription Factors, Adaptive Immunity|
|Pathways||Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation|
Application DetailsProduct Details anti-GATA3 Antibody Target Details GATA3 Handling References for anti-GATA3 antibody (ABIN967621) Images back to top
Methanol Procedure for a 96-well plate, with nuclear counterstain:
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate , and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or fixation buffer to each well and incubating for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells by adding 100 µl of -20°C 90% methanol to each well and incubating for 5 minutes at RT.
4. Remove the permeabilizer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 µl of blocking buffer (3% FBS in 1× PBS) or to each well and incubating for 30 minutes at RT.
6. Remove the blocking buffer, dilute the antibody in blocking buffer or Stain Buffer (FBS), and stain the cells by adding 50 µl of the diluted antibody to each well and incubating for 1 hour at RT.
7. Remove the diluted antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, dilute the second-step reagent in blocking buffer or Stain Buffer (FBS), and stain the cells by adding 50 µl of the diluted second-step reagent to each well and incubating for 1 hour at RT.
9. Remove the diluted second-step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 100 ml of a 2 mg/ml solution of Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-GATA3 Antibody Target Details GATA3 Application Details References for anti-GATA3 antibody (ABIN967621) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-GATA3 antibody (ABIN967621)Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Handling Images back to top
|Product cited in:||
Kouros-Mehr, Slorach, Sternlicht, Werb: "GATA-3 maintains the differentiation of the luminal cell fate in the mammary gland." in: Cell, Vol. 127, Issue 5, pp. 1041-55, 2006
Usary, Llaca, Karaca, Presswala, Karaca, He, Langerød, Kåresen, Oh, Dressler, Lønning, Strausberg, Chanock, Børresen-Dale, Perou: "Mutation of GATA3 in human breast tumors." in: Oncogene, Vol. 23, Issue 46, pp. 7669-78, 2004
Steenbergen, OudeEngberink, Kramer, Schrijnemakers, Verheijen, Meijer, Snijders: "Down-regulation of GATA-3 expression during human papillomavirus-mediated immortalization and cervical carcinogenesis." in: The American journal of pathology, Vol. 160, Issue 6, pp. 1945-51, 2002
Van Esch, Groenen, Nesbit, Schuffenhauer, Lichtner, Vanderlinden, Harding, Beetz, Bilous, Holdaway, Shaw, Fryns, Van de Ven, Thakker, Devriendt: "GATA3 haplo-insufficiency causes human HDR syndrome." in: Nature, Vol. 406, Issue 6794, pp. 419-22, 2000
Zheng, Flavell: "The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells." in: Cell, Vol. 89, Issue 4, pp. 587-96, 1997
Yang, Gu, Romeo, Bories, Motohashi, Yamamoto, Engel: "Human GATA-3 trans-activation, DNA-binding, and nuclear localization activities are organized into distinct structural domains." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2201-12, 1994
Marine, Winoto: "The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7284-8, 1991
Anderson, Holzer: "Exercise-induced asthma: is it the right diagnosis in elite athletes?" in: The Journal of allergy and clinical immunology, Vol. 106, Issue 3, pp. 419-28, 2000
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