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anti-Rat (Rattus) GATA3 antibody for Enzyme Immunoassay

Recommended GATA3 Antibody (supplied by: Log in to see )

Antigen
GATA Binding Protein 3 (GATA3) Antibodies
  • cb550
  • GATA-3
  • Gata-3
  • gata3
  • GATA3
  • gta3
  • HDR
  • hdr
  • HDRS
  • jal
  • xGATA-3
  • xgata-3
  • XGATA3
Epitope
N-Term
23
21
20
16
15
13
13
8
7
6
5
4
3
3
3
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
201
102
41
10
10
8
6
6
5
2
1
1
1
Host
Goat
118
64
19
14
Clonality
Polyclonal
Conjugate
This GATA3 antibody is un-conjugated
8
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
151
87
62
30
24
24
23
19
6
5
2
1
1
1
Supplier
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Catalog No. ABIN401530
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088685245 ABIN499877 EIA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00088685245 ABIN615854 EIA IHC (p) WB Goat N-Term Log in to see Polyclonal

Top referenced anti-GATA3 antibody for Enzyme Immunoassay

Similar anti-GATA3 Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine) Mouse (Murine) Pig (Porcine) Rat (Rattus)
Western Blotting (WB) 140 Antibodies 11 Antibodies 7 Antibodies 53 Antibodies 11 Antibodies 31 Antibodies
Intracellular Staining (ICS) 6 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies 3 Antibodies 3 Antibodies 15 Antibodies 3 Antibodies 10 Antibodies
Immunohistochemistry (IHC) 63 Antibodies 6 Antibodies 4 Antibodies 29 Antibodies 6 Antibodies 18 Antibodies

General

Antigen GATA Binding Protein 3 (GATA3) Antibodies
Epitope N-Term
(23), (21), (20), (16), (15), (13), (13), (8), (7), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(201), (102), (41), (10), (10), (8), (6), (6), (5), (2), (1), (1), (1)
Host Goat
(118), (64), (19), (14)
Clonality
Polyclonal
Conjugate This GATA3 antibody is un-conjugated
(8), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(151), (87), (62), (30), (24), (24), (23), (19), (6), (5), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-GATA3 Antibody

Target Details GATA3 Application Details Handling References for anti-GATA3 Antibody (ABIN401530) Images
Sequence EVTADQPRWV SHH-C
Specificity This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3

Target Details GATA3

Product Details anti-GATA3 Antibody Application Details Handling References for anti-GATA3 Antibody (ABIN401530) Images back to top
Antigen
Alternative Name GATA3 (GATA3 Antibody Abstract)
Background The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
Gene ID 2625
NCBI Accession NP_001002295
UniProt P23771
Research Area Lineage Markers, Transcription Factors, Adaptive Immunity
Pathways TCR Signaling

Application Details

Product Details anti-GATA3 Antibody Target Details GATA3 Handling References for anti-GATA3 Antibody (ABIN401530) Images back to top
Application Notes Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details References for anti-GATA3 Antibody (ABIN401530) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-GATA3 Antibody (ABIN401530)

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Handling Images back to top
Background publications

Van Opstal, Hepp, Suzuki, Henn: "Role of monkey nucleus reticularis tegmenti pontis in the stabilization of Listing's plane." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 22, pp. 7284-96, 1996

Marine, Winoto: "The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7284-8, 1991

Images

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Handling References for anti-GATA3 Antibody (ABIN401530) back to top
Supplier Images
 image for anti-GATA3 antibody (GATA Binding Protein 3) (N-Term) (ABIN401530) anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody
 image for anti-GATA3 antibody (GATA Binding Protein 3) (N-Term) (ABIN401530) anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody (Image 2)