anti-Baboon HLA-ABC antibody for Immunoprecipitation

Recommended HLA-ABC Antibody (supplied by: Log in to see )

Antigen
HLA-ABC Antibodies
  • HLAA
  • major histocompatibility complex, class I, A
  • HLA-A
Reactivity
Baboon, Cow (Bovine), Human, Primate
149
39
34
23
18
16
15
15
9
8
8
4
4
3
3
Host
Mouse
128
18
4
Clonality
Monoclonal
Conjugate
Un-conjugated
16
14
11
5
4
4
4
3
3
3
3
3
3
3
3
2
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
124
54
34
29
26
25
23
14
6
2
2
1
1
1
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Catalog No. ABIN255795
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN305205 FACS IHC (fro) IP ELISA Mouse IgG2a Log in to see W6-32
1 ABIN2474046 IHC (fro) FACS IF IP ELISA Mouse IgG2a Log in to see W6-32 4
1 ABIN2474054 Func IHC (fro) FACS IF IP ELISA Mouse IgG2a Log in to see W6-32 4
1 ABIN4318597 ELISA FACS Func ICC IF IHC IHC (fro) IP PerCP Mouse IgG2a Log in to see W6-32
1 ABIN292517 FACS IHC (fro) IP WB Mouse IgG2a, kappa Log in to see W6-32
1 ABIN1822554 Inhibition BR FACS IF IP IHC WB Mouse IgG2a, kappa Log in to see W6-32
1 ABIN601549 IHC (fro) FACS IP ELISA Mouse IgG2a Log in to see

General

Antigen HLA-ABC Antibodies
Reactivity Baboon, Cow (Bovine), Human, Primate
(149), (39), (34), (23), (18), (16), (15), (15), (9), (8), (8), (4), (4), (3), (3)
Host Mouse
(128), (18), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(16), (14), (11), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
(124), (54), (34), (29), (26), (25), (23), (14), (6), (2), (2), (1), (1), (1)
Pubmed 20 references available
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Product details

Target details Application Details Handling References Images
Specificity This antibody recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Purification Protein G purified
Immunogen Purified human tonsil lymphocyte membranes.
Clone W6-32
Isotype IgG2a

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name HLA ABC (HLA-ABC Antibody Abstract)
Background Gene Symbol: HLA-A
Gene ID 3105
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Application Details

Product details Target details Handling References Images back to top
Application Notes Flow Cytometry 1:50-1:100, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, FunctionalAntibody does not work in Immunohistochemistry-Paraffin.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References

Product details Target details Application Details Handling Images back to top
Product cited in:

Jones, Kosmoliaptsis, Apps, Lapaque, Smith, Kono, Chang, Boyle, Taylor, Trowsdale, Allen: "HLA class I allelic sequence and conformation regulate leukocyte Ig-like receptor binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 2990-7, 2011

Zuo, Quinn, Tamblyn, Thomas, Feederle, Delecluse, Hislop, Rowe et al.: "The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the ..." in: Journal of virology, Vol. 85, Issue 4, pp. 1604-14, 2011

Spentzou, Bergin, Gill, Cheeseman, Ashraf, Kaltsidis, Cashin-Cox, Anjarwalla, Steel, Higgs, Pozniak, Piechocka-Trocha, Wong, Anzala, Karita, Dally, Gotch, Walker, Gilmour, Hayes: "Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates." in: The Journal of infectious diseases, Vol. 201, Issue 5, pp. 720-9, 2010

Hinrichs, Föll, Bade-Döding, Huyton, Blasczyk, Eiz-Vesper: "The nature of peptides presented by an HLA class I low expression allele." in: Haematologica, Vol. 95, Issue 8, pp. 1373-80, 2010

Narita, Masuko, Kurasaki, Kitajima, Takenouchi, Saitoh, Watanabe, Furukawa, Toba, Fuse, Aizawa, Kawakami, Oka, Sugiyama, Takahashi: "WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase." in: International journal of medical sciences, Vol. 7, Issue 2, pp. 72-81, 2010

Fujita, Abe, Inokuma, Sasaki, Hoshina, Natsuga, Nishie, McMillan, Nakamura, Shimizu, Akiyama, Sawamura, Shimizu: "Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 32, pp. 14345-50, 2010

Vitadello, Doria, Tarricone, Ghirardello, Gorza: "Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation." in: Arthritis research & therapy, Vol. 12, Issue 2, pp. R52, 2010

Grotzke, Harriff, Siler, Nolt, Delepine, Lewinsohn, Lewinsohn: "The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle." in: PLoS pathogens, Vol. 5, Issue 4, pp. e1000374, 2009

Koene, Arts-Hilkes, van Dijk, van der Ven, Slootweg, de Weger, Tilanus: "High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss." in: Human immunology, Vol. 65, Issue 12, pp. 1455-62, 2004

Dressel, Grzeszik, Kreiss, Lindemann, Herrmann, Walter, Günther: "Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes." in: Cancer research, Vol. 63, Issue 23, pp. 8212-20, 2003

Ishitani, Sageshima, Lee, Dorofeeva, Hatake, Marquardt, Geraghty: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003

Goldman-Wohl, Ariel, Greenfield, Hochner-Celnikier, Lavy, Yagel: "A study of human leukocyte antigen G expression in hydatidiform moles." in: American journal of obstetrics and gynecology, Vol. 185, Issue 2, pp. 476-80, 2001 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro))

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Verbeek, Westphal, Ruiter, de Waal: "T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5876-84, 1995

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994

Tanabe, Sekimata, Ferrone, Takiguchi: "Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 10, pp. 3202-9, 1992

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987

Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." in: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, 1986

Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." in: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, 1982

Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, 1978

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Analysis using the PE/Cy5 co...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Analysis using the FITC conj...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Staining of human peripheral...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Staining of human peripheral...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Staining of human peripheral...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Staining of human peripheral...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Staining of human peripheral...
Flow Cytometry (FACS) image for anti-HLA-ABC (HLA-ABC) antibody (ABIN255795) Flow Cytometry: HLA ABC Antibody (W6/32) [ABIN2557955] - Human lymphoblast. Image pro...