anti-Human IL17A antibody for Functional Studies

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • Il17
  • IL17A
  • CTLA8
  • IL-17
  • IL-17A
  • IL17
  • Ctla-8
  • Ctla8
  • CTLA-8
  • ChIL-17
  • IL-17F
  • interleukin 17A
  • interleukin 17A-like
  • interleukin 17F
  • Il17a
  • IL17A
  • LOC100339322
  • IL17F
Reactivity
Human
373
133
41
7
7
5
3
3
2
2
Host
Rabbit
248
133
77
40
3
3
Clonality
Polyclonal
Conjugate
This IL17A antibody is un-conjugated
56
32
27
20
16
15
15
12
11
11
10
7
7
7
7
7
5
5
5
4
4
4
4
4
4
3
2
2
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
269
229
195
44
30
19
19
15
8
8
8
7
6
6
6
4
4
4
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN116119
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116120 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1107806 Func Mouse IgG2b Log in to see B-B51
1 ABIN1724553 ELISpot Func ELISA Mouse IgG1 Log in to see B-B51
1 ABIN208854 Func ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN786721 ELISA (Capture) ELISA (Detection) Func FACS Neut Mouse IgG1 Log in to see 412G6-06

General

Antigen Interleukin 17A (IL17A) Antibodies
Reactivity Human
(373), (133), (41), (7), (7), (5), (3), (3), (2), (2)
Host Rabbit
(248), (133), (77), (40), (3), (3)
Clonality
Polyclonal
Conjugate This IL17A antibody is un-conjugated
(56), (32), (27), (20), (16), (15), (15), (12), (11), (11), (10), (7), (7), (7), (7), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(269), (229), (195), (44), (30), (19), (19), (15), (8), (8), (8), (7), (6), (6), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling Images
Specificity This antibody reacts with Interleukin-17 (IL-17).
Purification Affinity Chromatography.
Immunogen Highly pure (>98%) recombinant hIL-17A (human Interleukin-17A).

Target Details IL17A

Product Details anti-IL17A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-17A (IL17A) (IL17A Antibody Abstract)
Background IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surface expression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5 % amino acid homology to mouse IL17 and 58 % amino acid homology to rat IL17.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17
Gene ID 3605
NCBI Accession NP_002181
UniProt Q16552
Research Area Cytokines, Receptors, Inflammation, Immunology, Virology, Cancer

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman IL-17A (50.00 ng/mL), a concentration of 0.9-1.3 μg/mL of previous lots of thisantibody is required. Immunohistochemistry on Parrafin Sections: 0.25 μg/mL, Overnight at 4 °C. This antibody stained sections of Human breast invasive ductal carcinoma. An HRP-labeledpolymer detection system was used with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Sandwich ELISA: To detect Human IL-17A by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Human IL-17A (PP1037B1 orPP1037B2) as Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinantHuman IL-17A. Western Blot: To detect Human IL-17A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human IL-17A is 1.5-3.0 ng/lane, under either
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or at -20 °C for longer. This product should be stored undiluted.