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anti-Human IL17A antibody for Immunohistochemistry (Frozen Sections)

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • ChIL-17
  • Ctla-8
  • CTLA-8
  • CTLA8
  • Ctla8
  • IL-17
  • IL-17A
  • IL-17F
  • IL17
  • Il17
  • IL17A
Epitope
N-Term
30
14
14
14
13
13
11
11
8
7
6
6
4
4
4
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
305
173
53
10
5
5
1
Host
Rabbit
200
128
96
42
8
3
1
Clonality
Polyclonal
Conjugate
This IL17A antibody is un-conjugated
63
31
26
18
17
9
7
6
6
6
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
251
249
138
42
39
27
24
22
19
15
11
9
9
9
9
8
7
6
5
4
4
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN493065
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN281663 ELISA (Capture) ELISPOT (Capture) FACS IHC (fro) IP ICS Mouse IgG1 kappa Log in to see 64CAP17 1
0.00088051456 ABIN1859349 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 20-155 Log in to see Polyclonal
0.00088051456 ABIN281666 IHC (fro) ELISA Mouse IgG1 kappa Log in to see 64DEC17

Top referenced anti-IL17A antibody for Immunohistochemistry (Frozen Sections)

Similar anti-IL17A Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 172 Antibodies 84 Antibodies 26 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Neutralization (Neut) 30 Antibodies 8 Antibodies 1 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Intracellular Staining (ICS) 11 Antibodies 9 Antibodies 13 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 5 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies 8 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 12 Antibodies

General

Antigen Interleukin 17A (IL17A) Antibodies
Epitope N-Term
(30), (14), (14), (14), (13), (13), (11), (11), (8), (7), (6), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(305), (173), (53), (10), (5), (5), (1)
Host Rabbit
(200), (128), (96), (42), (8), (3), (1)
Clonality
Polyclonal
Conjugate This IL17A antibody is un-conjugated
(63), (31), (26), (18), (17), (9), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(251), (249), (138), (42), (39), (27), (24), (22), (19), (15), (11), (9), (9), (9), (9), (8), (7), (6), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN493065) Images
Sequence NSV PIQ QEI LVL RE PPH C
Specificity This antibody detects a single 18 kDa of IL17. It does not cross reacts with other members of the pro-inflammtory cytokines or with other leukotrienes tested and also does not label any other cellular protein on western blots.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography
Immunogen Synthetic peptide selective for IL17 from near extra-cytoplasmic domain peptide sequence from N-terminal

Target Details IL17A

Product Details anti-IL17A Antibody Application Details Handling References for anti-IL17A Antibody (ABIN493065) Images back to top
Antigen
Alternative Name Interleukin-17A (IL17A) (IL17A Antibody Abstract)
Background The primary function of the body defense mechanism/immune system is to protect the organism from invading pathogens. In this regard a very effective and dynamic cellular network has evolved that comprise of innate and adaptive immunity. Most components of innate immunity are present before the onset of infection and constitute cellular and molecular components that recognize classes of molecules peculiar to pathogens. In contrast, adaptive immunity responds to the challenge with a high degree of specificity as well as the remarkable property of memory. Although adaptive immune responses are the most effective to clear invading pathogens (1, 2), innate immune responses should be precedent of activation of adaptive immune cells (2). In other words, adaptive immune responses can not arise without help of innate immune responses. Antigen presenting cells (APCs) are key initiators of immune responses. During infection various cytochines are released and via activation of various TLR receptor family, they synthesized various interleukins. IL17 gene was mapped at a single site on mouse chromosome 1A and human chromosome 2q31 (2). Pro inflammatory cytokine, IL-17 (IL-17A or CTLA-8) plays a central role in inflammation and autoimmunity. The iIL17, like other cytokines is produce by activation of T cell receptor through CD3 cross linking (1). IL-17 is a crucial effector cytokine, whose production is specifically triggered by IL-23, and it has been shown to be an essential inflammatory mediator in other autoimmune diseases and inflammatory conditions. In human the co-stimulation of T cells thru CD28 several costimulatory molecules (ICOS, 4-1BB, CD40L) mildly enhance the IL17 expression where as IL23 profoundly enhance the CD3 induced IL17 expression. The IL-17 expression is also sensitive to cyclosporin-A and MAPK inhibitors, suggesting the involvement of the calcineurin/NFAT and MAPK signaling pathways. The predicted 155-amino acids sequence contains an N-terminal signal peptide and exhibits 72 % amino acid identity with HVS13, an open reading frame from a T-lymphotropic Herpesvirus saimiri, and 63 % with murine CTLA8 (3). Based on hydropathy plot has 2 transmembrane domains (TMD) at N and C-terminal end of the protein.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17
Gene ID 3605
NCBI Accession NP_002181
UniProt Q16552
Research Area Cytokines, Receptors, Inflammation, Immunology, Virology, Cancer
Pathways TCR Signaling

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN493065) Images back to top
Application Notes Immunohistochemistry on frozen sections: 1: 200. Western blot > 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN493065) Images back to top
Concentration 0.65 - 0.95 mg/mL
Buffer Stabilization buffer with 0.02 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-IL17A Antibody (ABIN493065)

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Background publications

Huang, Lu, Li, Xu, Yi, Chen: "Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion." in: World journal of gastroenterology : WJG, Vol. 12, Issue 34, pp. 5483-9, 2006

Fossiez, Djossou, Chomarat, Flores-Romo, Ait-Yahia, Maat, Pin, Garrone, Garcia, Saeland, Blanchard, Gaillard, Das Mahapatra, Rouvier, Golstein, Banchereau, Lebecque: "T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines." in: The Journal of experimental medicine, Vol. 183, Issue 6, pp. 2593-603, 1996

Yao, Painter, Fanslow, Ulrich, Macduff, Spriggs, Armitage: "Human IL-17: a novel cytokine derived from T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 12, pp. 5483-6, 1996

Rouvier, Luciani, Mattéi, Denizot, Golstein: "CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5445-56, 1993