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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||3 Antibodies|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Blocking Reagent (BR)||2 Antibodies|
|Confocal Microscopy (CM)||2 Antibodies|
|Depletion (Dep)||1 Antibodies|
|ELISA (Capture)||7 Antibodies|
|ELISA (Detection)||8 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||78 Antibodies|
|Functional Studies (Func)||9 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies|
|Antigen||Interleukin 17A (IL17A) Antibodies|
|Conjugate||This IL17A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Neutralization (Neut), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-IL17A AntibodyTarget Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN401101) Images
|Specificity||This antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids.|
|Immunogen||Recombinant human IL17-A protein|
Target Details IL17AProduct Details anti-IL17A Antibody Application Details Handling References for anti-IL17A Antibody (ABIN401101) Images back to top
|Alternative Name||Interleukin-17A (IL17A) (IL17A Antibody Abstract)|
|Background||IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8 in rodents) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NFkappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL-17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17|
|Research Area||Cytokines, Receptors, Inflammation, Immunology, Virology, Cancer|
Application DetailsProduct Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN401101) Images back to top
ELISA: 1/20000 - 1/100000. Western Blot: 1/500 - 1/2000. Neutralization: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN401101) Images back to top
|Reconstitution||Restore with 0.1 mL of deionized water (or equivalent).|
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IL17A Antibody (ABIN401101)Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Yin, Wen, Li et al.: "Hypoxia enhances stimulating effect of amyloid beta peptide (25-35) for interleukin 17 and T helper lymphocyte subtype 17 upregulation in cultured peripheral blood mononuclear cells." in: Microbiology and immunology, Vol. 53, Issue 5, pp. 281-6, 2009 (PubMed).
Shin, Shin, Noh: "Interleukin-17A inhibits adipocyte differentiation in human mesenchymal stem cells and regulates pro-inflammatory responses in adipocytes." in: Biochemical pharmacology, Vol. 77, Issue 12, pp. 1835-44, 2009 (PubMed).
Paust, Turner, Steinmetz et al.: "The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 5, pp. 969-79, 2009 (PubMed).
ImagesProduct Details anti-IL17A Antibody Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN401101) back to top