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anti-Mouse (Murine) IL17A antibody for Intracellular Staining

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • Il17
  • IL17A
  • CTLA8
  • IL-17
  • IL-17A
  • IL17
  • Ctla-8
  • Ctla8
  • CTLA-8
  • ChIL-17
  • IL-17F
  • interleukin 17A
  • interleukin 17A-like
  • interleukin 17F
  • Il17a
  • IL17A
  • LOC100339322
  • IL17F
Reactivity
Mouse (Murine)
425
185
65
11
7
5
3
2
2
2
1
Host
Rat
244
199
94
51
8
3
1
Clonality
Monoclonal
Conjugate
This IL17A antibody is un-conjugated
66
36
33
24
17
15
15
12
10
10
8
7
7
7
7
7
7
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
Application
Intracellular Staining (ICS), Neutralization (Neut), ELISA
340
304
200
63
40
35
26
21
16
14
12
9
8
7
6
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1177292
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Top referenced anti-IL17A antibody for Intracellular Staining

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Application / Reactivity Mouse (Murine)
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Interleukin 17A (IL17A) Antibodies
Reactivity Mouse (Murine)
(425), (185), (65), (11), (7), (5), (3), (2), (2), (2), (1)
Host Rat
(244), (199), (94), (51), (8), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL17A antibody is un-conjugated
(66), (36), (33), (24), (17), (15), (15), (12), (10), (10), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Intracellular Staining (ICS), Neutralization (Neut), ELISA
(340), (304), (200), (63), (40), (35), (26), (21), (16), (14), (12), (9), (8), (7), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN1177292) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Recombinant Mouse IL-17A Protein
Clone TC11-18H10
Isotype IgG1, kappa

Target Details IL17A

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Antigen
Alternative Name IL-17A (IL17A Antibody Abstract)
Background The TC11-18H10 antibody reacts with recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro, it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRalphabeta+CD4-CD8- thymocytes. Functional studies of TC11-18H10 mAb by neutralization of mouse IL-17A bioactivity. Recombinant mouse IL-17A (2 ng/well) was pre-incubated with various amounts of TC11-18H10 mAb or medium as a control in a 96-well plate for 1 hour at 37°C. Following the incubation, NIH-3T3 cells were added at 5x10^4 cells per well and incubated at 37°C. After 24 hours of incubation, the supernatant was harvested and the concentration of IL-6 was quantified using BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301).
Synonyms: Interleukin-17A, Il17a, Cytotoxic T-lymphocyte-associated antigen, CTLA-8
Pathways TCR Signaling

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN1177292) Images back to top
Protocol This procedure is to be used for blocking, it is not routinely tested for each batch. 1. In the appropriate tissue culture medium dilute TC11-18H10 NA/LE mAb with the highest concentration of 12 µg/ml at a 1:4 series dilution in the tissue culture hood for a total of 7 different concentrations.
2. Add 50 µl into each well. For the negative control, add 50 µl tissue culture medium.
3. Set the triplicate for each testing concentration of TC11-18H10 NA/LE antibody . 560268 4. Dilute the rmIL-17A in the tissue culture medium to 40 ng/ml. Add 50 µl into each well. Tap the plate to mix the mAb and rmIL-17A together.
5. Incubate the plate in 37°C, 5% CO2 incubator for 1 hour.
6. Count the log phase NIH-3T3 cells and dilute the cells to 5x10^5 cell/ml. Seed 100 µl cells into each well.
7. Incubate the cells in 37°C, 5% CO2 incubator for 24 hours. 8. Harvest the supernantant. Check the IL-6 concentration from each well using the BD Cytometric Bead Array Mouse IL-6 Flex Set.
9. Calculate the IL-6 concentration of each well from the standard curve. Calculate the mean of each concentration triplicate and plot a chart
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN1177292) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL17A Antibody (ABIN1177292)

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Product cited in:

Yen, Cheung, Scheerens, Poulet, McClanahan, McKenzie, Kleinschek, Owyang, Mattson, Blumenschein, Murphy, Sathe, Cua, Kastelein, Rennick: "IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6." in: The Journal of clinical investigation, Vol. 116, Issue 5, pp. 1310-6, 2006

Schwarzenberger, La Russa, Miller, Ye, Huang, Zieske, Nelson, Bagby, Stoltz, Mynatt, Spriggs, Kolls: "IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6383-9, 1998

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Images

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Supplier Images
ELISA image for anti-IL17A antibody (Interleukin 17A) (ABIN1177292) anti-Interleukin 17A (IL17A) antibody