|Antigen||Interleukin 17A (IL17A) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL17A antibody is un-conjugated Alternatives|
Intracellular Staining (ICS), Neutralization (Neut), ELISA
|3 references available|
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Product Details anti-IL17A AntibodyTarget Details IL17A Application Details Handling ProductDetails: References for anti-IL17A antibody (ABIN1177292) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||Recombinant Mouse IL-17A Protein|
Target Details IL17AProduct Details anti-IL17A Antibody Application Details Handling ProductDetails: References for anti-IL17A antibody (ABIN1177292) Images back to top
|Alternative Name||IL-17A (IL17A Antibody Abstract)|
The TC11-18H10 antibody reacts with recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro, it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRalphabeta+CD4-CD8- thymocytes. Functional studies of TC11-18H10 mAb by neutralization of mouse IL-17A bioactivity. Recombinant mouse IL-17A (2 ng/well) was pre-incubated with various amounts of TC11-18H10 mAb or medium as a control in a 96-well plate for 1 hour at 37°C. Following the incubation, NIH-3T3 cells were added at 5x10^4 cells per well and incubated at 37°C. After 24 hours of incubation, the supernatant was harvested and the concentration of IL-6 was quantified using BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301).
Synonyms: Interleukin-17A, Il17a, Cytotoxic T-lymphocyte-associated antigen, CTLA-8
Application DetailsProduct Details anti-IL17A Antibody Target Details IL17A Handling ProductDetails: References for anti-IL17A antibody (ABIN1177292) Images back to top
This procedure is to be used for blocking, it is not routinely tested for each batch. 1. In the appropriate tissue culture medium dilute TC11-18H10 NA/LE mAb with the highest concentration of 12 µg/ml at a 1:4 series dilution in the tissue culture hood for a total of 7 different concentrations.
2. Add 50 µl into each well. For the negative control, add 50 µl tissue culture medium.
3. Set the triplicate for each testing concentration of TC11-18H10 NA/LE antibody . 560268 4. Dilute the rmIL-17A in the tissue culture medium to 40 ng/ml. Add 50 µl into each well. Tap the plate to mix the mAb and rmIL-17A together.
5. Incubate the plate in 37°C, 5% CO2 incubator for 1 hour.
6. Count the log phase NIH-3T3 cells and dilute the cells to 5x10^5 cell/ml. Seed 100 µl cells into each well.
7. Incubate the cells in 37°C, 5% CO2 incubator for 24 hours. 8. Harvest the supernantant. Check the IL-6 concentration from each well using the BD Cytometric Bead Array Mouse IL-6 Flex Set.
9. Calculate the IL-6 concentration of each well from the standard curve. Calculate the mean of each concentration triplicate and plot a chart
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL17A Antibody Target Details IL17A Application Details ProductDetails: References for anti-IL17A antibody (ABIN1177292) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
ProductDetails: References for anti-IL17A antibody (ABIN1177292)Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
|Product cited in:||
Yen, Cheung, Scheerens, Poulet, McClanahan, McKenzie, Kleinschek, Owyang, Mattson, Blumenschein, Murphy, Sathe, Cua, Kastelein, Rennick: "IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6." in: The Journal of clinical investigation, Vol. 116, Issue 5, pp. 1310-6, 2006
Schwarzenberger, La Russa, Miller, Ye, Huang, Zieske, Nelson, Bagby, Stoltz, Mynatt, Spriggs, Kolls: "IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6383-9, 1998
Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996
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