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anti-Mouse (Murine) IL17A antibody for Blocking Antibody

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • Il17
  • IL17A
  • CTLA8
  • IL-17
  • IL-17A
  • IL17
  • Ctla-8
  • Ctla8
  • CTLA-8
  • ChIL-17
  • IL-17F
  • interleukin 17A
  • interleukin 17A-like
  • interleukin 17F
  • Il17a
  • IL17A
  • LOC100339322
  • IL17F
Reactivity
Mouse (Murine)
425
185
65
11
7
5
3
2
2
2
1
Host
Rat
244
199
94
51
8
3
1
Clonality
Monoclonal
Conjugate
This IL17A antibody is un-conjugated
66
36
33
24
17
15
15
12
10
10
8
7
7
7
7
7
7
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
Application
Blocking Antibody (Inhibition), Neutralization (Neut), ELISA, Western Blotting (WB)
339
304
200
63
40
35
26
21
16
14
12
9
8
7
6
5
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2689741
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689742 Inhibition Neut ELISA WB Rat IgG1, kappa Log in to see TC11
1 ABIN636682 Inhibition ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-IL17A antibody for Blocking Antibody

Similar anti-IL17A Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Cell Culture (CC) 1 Antibodies
Confocal Microscopy (CM) 2 Antibodies
ELISA 101 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 83 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Interleukin 17A (IL17A) Antibodies
Reactivity Mouse (Murine)
(425), (185), (65), (11), (7), (5), (3), (2), (2), (2), (1)
Host Rat
(244), (199), (94), (51), (8), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL17A antibody is un-conjugated
(66), (36), (33), (24), (17), (15), (15), (12), (10), (10), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Blocking Antibody (Inhibition), Neutralization (Neut), ELISA, Western Blotting (WB)
(339), (304), (200), (63), (40), (35), (26), (21), (16), (14), (12), (9), (8), (7), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN2689741) Images
Brand BD Pharmingen™
Characteristics The TC11-18H10 antibody reacts with recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro, it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRαβ+CD4-CD8-thymocytes. Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug™ (1 μg/mL final concentration, Cat. No. 555029) without (left panel) or with PMA (5 ng/mL final concentration, Sigma, Cat. No. P-8139) and Ionomycin (500 ng/mL final concentration, Sigma, Cat. No.I-0634) (middle and right panels). The cells were fixed, permeabilized and subsequently stained with 0.125 μg of PE conjugated anti-mouse IL-17A (PE-TC11-18H10, Cat. No.559502) using BD Pharmingen I/C staining protocol (left and middle panels) . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody (10 μg, right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained (left panel) and unlabeled antibody blocking (right panel) controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-17A - Purified - Clone TC11-18H10 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant Mouse IL-17 Protein
Clone TC11
Isotype IgG1, kappa

Target Details IL17A

Product Details anti-IL17A Antibody Application Details Handling References for anti-IL17A Antibody (ABIN2689741) Images back to top
Antigen
Alternative Name IL-17A (IL17A Antibody Abstract)
Pathways TCR Signaling

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN2689741) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN2689741) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL17A Antibody (ABIN2689741)

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Product cited in:

Schwarzenberger, La Russa, Miller, Ye, Huang, Zieske, Nelson, Bagby, Stoltz, Mynatt, Spriggs, Kolls: "IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6383-9, 1998

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996