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anti-Rat (Rattus) IL17A antibody for Flow Cytometry

Recommended IL17A Antibody (supplied by: Log in to see )

Antigen
Interleukin 17A (IL17A) Antibodies
  • ChIL-17
  • Ctla-8
  • CTLA-8
  • CTLA8
  • Ctla8
  • IL-17
  • IL-17A
  • IL-17F
  • IL17
  • Il17
  • IL17A
Reactivity
Mouse (Murine), Rat (Rattus)
306
173
53
10
5
5
1
Host
Rat
201
128
95
42
8
3
1
Clonality
Monoclonal
Conjugate
This IL17A antibody is conjugated to PE-Cy7
63
31
26
18
17
9
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
252
249
137
42
39
27
23
22
19
15
11
10
9
9
9
8
7
6
5
4
4
2
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2680348
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN2680350 FACS ICS EFluor 450 Rat IgG2a kappa Log in to see 17B7
0.3068567 ABIN2680354 FACS ICS PE-eFluor® 610 Rat IgG2a kappa Log in to see 17B7
0.3068567 ABIN2680352 FACS ICS EFluor 660 Rat IgG2a kappa Log in to see 17B7
0.0010666476 ABIN335850 FACS ICS PerCP-Cy5.5 Rat IgG2a kappa Log in to see 17B7 1
0.0010666476 ABIN286632 FACS ICS Alexa Fluor 488 Rat IgG2a kappa Log in to see 17B7 1
0.0010666476 ABIN2568239 FACS FITC Rat IgG2a kappa Log in to see 17B7
0.0010666476 ABIN2568240 FACS PE Rat IgG2a kappa Log in to see 17B7
0.0010666476 ABIN282870 FACS ICS APC Rat IgG2a kappa Log in to see 17B7
0.0010666476 ABIN279136 FACS ICS PE Rat IgG2a kappa Log in to see 17B7
0.0010666476 ABIN278173 FACS ICS FITC Rat IgG2a kappa Log in to see 17B7
0.0010666476 ABIN2568238 FACS FITC Rat IgG2a kappa Log in to see 17B7

Top referenced anti-IL17A antibody for Flow Cytometry

Similar anti-IL17A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Confocal Microscopy (CM) 2 Antibodies 2 Antibodies
ELISA 19 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 26 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies

General

Antigen Interleukin 17A (IL17A) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(306), (173), (53), (10), (5), (5), (1)
Host Rat
(201), (128), (95), (42), (8), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL17A antibody is conjugated to PE-Cy7
(63), (31), (26), (18), (17), (9), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(252), (249), (137), (42), (39), (27), (23), (22), (19), (15), (11), (10), (9), (9), (9), (8), (7), (6), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL17A Antibody

Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN2680348) Images
Specificity The antibody reacts with mouse and rat IL-17A with no recognition of IL-17F. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells, the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Characteristics The eBio17B7 antibody reacts with mouse and rat IL-17A with no recognition of IL-17F. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells, the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T- lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL- 17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor. IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Synonyms: Synonyms: Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
Clone 17B7
Isotype IgG2a kappa

Target Details IL17A

Product Details anti-IL17A Antibody Application Details Handling References for anti-IL17A Antibody (ABIN2680348) Images back to top
Antigen
Alternative Name IL-17A (IL17A Antibody Abstract)
Pathways TCR Signaling

Application Details

Product Details anti-IL17A Antibody Target Details IL17A Handling References for anti-IL17A Antibody (ABIN2680348) Images back to top
Application Notes Applications reported: This eBio17B7 antibody has been reported for use intracellular satining followed by flow cytometric analysis.

Applications tested: This eBio17B7 antibody has been tested by intracellular staining and flow cytometric analysis of PMA and Ionomycin-restimulated splenocytes cultured under Th17-polarizing conditions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Staining has been successfully done using the Foxp3 buffer system (cat 00-5523). Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (100 μLcell sample + 100 μLIC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4 °C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Restrictions For Research Use only

Handling

Product Details anti-IL17A Antibody Target Details IL17A Application Details References for anti-IL17A Antibody (ABIN2680348) Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light- sensitive material. This tandem dye is sensitive to photo- induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-IL17A Antibody (ABIN2680348)

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling Images back to top
Product cited in:

Cao, Lan, Shi, Zhou, Liu, Liu, Tang, Qiu, Qiu, Xu, Fan, Liu: "Anti-IL-23 antibody blockade of IL-23/IL-17 pathway attenuates airway obliteration in rat orthotopic tracheal transplantation." in: International immunopharmacology, Vol. 11, Issue 5, pp. 569-75, 2011

Images

Product Details anti-IL17A Antibody Target Details IL17A Application Details Handling References for anti-IL17A Antibody (ABIN2680348) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL17A antibody (Interleukin 17A)  (PE-Cy7) (ABIN2680348) Intracellular staining of 10-day Th17-polarized mouse splenocytes either unstimulated...