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anti-Human ITK antibody for Western Blotting

Recommended ITK Antibody (supplied by: Log in to see )

Antigen
IL2-Inducible T-Cell Kinase (ITK) Antibodies
  • Emt
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • Tcsk
  • Tsk
Epitope
C-Term
21
20
9
9
9
8
8
7
5
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
102
24
17
3
1
1
1
1
Host
Goat
59
28
25
Clonality
Polyclonal
Conjugate
This ITK antibody is un-conjugated
7
6
6
6
6
6
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
94
72
7
6
6
6
6
4
3
1
1
Supplier
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Catalog No. ABIN184605
$ 318.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN969222 ICC FACS ELISA WB Mouse IgG1 Log in to see 5G6 2
0.13398378 ABIN1724708 ELISA WB Mouse IgG1 AA 2-110 Log in to see 5G12C4 2
0.13398378 ABIN967287 IP WB Mouse IgG1 AA 1-26 Log in to see 2F12 3
0.13398378 ABIN2855946 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.13398378 ABIN1539219 WB Rabbit Ig AA 25-54, N-Term Log in to see Polyclonal
0.13398378 ABIN1109424 EIA IF WB Mouse IgG1 Log in to see 5G6 1
0.13398378 ABIN392100 WB Rabbit Ig AA 169-198, Center Log in to see Polyclonal
0.13398378 ABIN1109423 EIA WB Mouse IgG1 Log in to see 5G12C4 3
0.13398378 ABIN2465858 ELISA WB Goat C-Term Log in to see Polyclonal
0.13398378 ABIN1873325 WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN256939 ELISA WB Goat IgG C-Term Log in to see Polyclonal
0.13398378 ABIN2490470 ELISA WB Mouse IgG1 AA 2-110 Log in to see 5G12C4
0.13398378 ABIN931475 ELISA WB Mouse IgG1 AA 2-110 Log in to see 5G12C4
0.13398378 ABIN517266 WB Mouse AA 1-620, full length Log in to see Polyclonal
0.13398378 ABIN2488581 WB Rabbit Ig Fraction AA 24-54, N-Term Log in to see Polyclonal
0.13398378 ABIN2488582 WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.13398378 ABIN561561 ELISA WB Mouse AA 1-111, partial Log in to see Polyclonal
0.13398378 ABIN1844114 ELISA WB Mouse IgG1 AA 2-110 Log in to see
0.13398378 ABIN1846240 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.13398378 ABIN2487501 WB Goat Log in to see Polyclonal

Top referenced anti-ITK antibody for Western Blotting

Similar anti-ITK Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 90 Antibodies

General

Antigen IL2-Inducible T-Cell Kinase (ITK) Antibodies
Epitope C-Term
(21), (20), (9), (9), (9), (8), (8), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(102), (24), (17), (3), (1), (1), (1), (1)
Host Goat
(59), (28), (25)
Clonality
Polyclonal
Conjugate This ITK antibody is un-conjugated
(7), (6), (6), (6), (6), (6)
Methode Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(94), (72), (7), (6), (6), (6), (6), (4), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-ITK Antibody

Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN184605) Images
Blocking Peptide Blocking peptide for this product available: ABIN369790
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.

Target Details ITK

Product Details anti-ITK Antibody Application Details Handling References for anti-ITK Antibody (ABIN184605) Images back to top
Antigen
Alternative Name ITK (ITK Antibody Abstract)
Background Synonyms: ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
Gene ID 3702, 16428
NCBI Accession NP_005537
Research Area Signaling, Tyrosine Kinases
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ITK Antibody Target Details ITK Handling References for anti-ITK Antibody (ABIN184605) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-ITK Antibody Target Details ITK Application Details References for anti-ITK Antibody (ABIN184605) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-ITK Antibody (ABIN184605)

Product Details anti-ITK Antibody Target Details ITK Application Details Handling Images back to top
Background publications

Tanaka, Asao, Ohtani, Nakamura, Sugamura: "A novel human tyrosine kinase gene inducible in T cells by interleukin 2." in: FEBS letters, Vol. 324, Issue 1, pp. 1-5, 1993

Images

Product Details anti-ITK Antibody Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN184605) back to top
Supplier Images
Western Blotting (WB) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (C-Term) (ABIN184605) ABIN184605 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). ...