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|Antigen||IL2-Inducible T-Cell Kinase (ITK) Antibodies|
|Epitope||AA 1-26 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ITK antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-ITK AntibodyTarget Details ITK Application Details Handling References for anti-ITK antibody (ABIN967287) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Fusion protein with aa.1-26 of human/mouse Itk|
Target Details ITKProduct Details anti-ITK Antibody Application Details Handling References for anti-ITK antibody (ABIN967287) Images back to top
|Alternative Name||ITK (ITK Antibody Abstract)|
|Background||Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)|
|Molecular Weight||72 kDa|
|Research Area||Signaling, Tyrosine Kinases|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-ITK Antibody Target Details ITK Handling References for anti-ITK antibody (ABIN967287) Images back to top
Related Products: ABIN968537
|Restrictions||For Research Use only|
HandlingProduct Details anti-ITK Antibody Target Details ITK Application Details References for anti-ITK antibody (ABIN967287) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-ITK antibody (ABIN967287)Product Details anti-ITK Antibody Target Details ITK Application Details Handling Images back to top
|Product cited in:||
Liu, Bunnell, Gurniak, Berg: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998
Heyeck, Wilcox, Bunnell, Berg: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997
Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993
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