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anti-Mouse (Murine) ITK antibody for Western Blotting

Recommended ITK Antibody (supplied by: Log in to see )

Antigen
IL2-Inducible T-Cell Kinase (ITK) Antibodies
  • Emt
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • Tcsk
  • Tsk
Epitope
AA 1-26
22
19
9
9
9
8
8
7
5
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
99
23
16
3
1
1
1
1
Host
Mouse
58
29
22
Clonality
Monoclonal
Conjugate
This ITK antibody is un-conjugated
7
6
6
6
6
6
Application
Immunoprecipitation (IP), Western Blotting (WB)
91
72
6
5
5
5
4
4
3
1
1
Supplier
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Catalog No. ABIN967287
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1873325 WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2487501 WB Goat Log in to see Polyclonal
0.0011806369 ABIN374095 WB Goat C-Term Log in to see Polyclonal 1
0.0011806369 ABIN680653 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN1087338 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN1087337 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN2563488 ELISA WB Goat IgG C-Term Log in to see Polyclonal
0.0011806369 ABIN2746783 ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN2746782 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN2419849 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN1868785 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-ITK antibody for Western Blotting

Similar anti-ITK Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 9 Antibodies 65 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 12 Antibodies
Dot Blot (DB) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunofluorescence (IF) 4 Antibodies

General

Antigen IL2-Inducible T-Cell Kinase (ITK) Antibodies
Epitope AA 1-26
(22), (19), (9), (9), (9), (8), (8), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(99), (23), (16), (3), (1), (1), (1), (1)
Host Mouse
(58), (29), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ITK antibody is un-conjugated
(7), (6), (6), (6), (6), (6)
Application Immunoprecipitation (IP), Western Blotting (WB)
(91), (72), (6), (5), (5), (5), (4), (4), (3), (1), (1)
Pubmed 3 references available
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Product Details anti-ITK Antibody

Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN967287) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Fusion protein with aa.1-26 of human/mouse Itk
Clone 2F12
Isotype IgG1

Target Details ITK

Product Details anti-ITK Antibody Application Details Handling References for anti-ITK Antibody (ABIN967287) Images back to top
Antigen
Alternative Name ITK (ITK Antibody Abstract)
Background Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
Molecular Weight 72 kDa
Research Area Signaling, Tyrosine Kinases
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ITK Antibody Target Details ITK Handling References for anti-ITK Antibody (ABIN967287) Images back to top
Comment

Related Products: ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-ITK Antibody Target Details ITK Application Details References for anti-ITK Antibody (ABIN967287) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-ITK Antibody (ABIN967287)

Product Details anti-ITK Antibody Target Details ITK Application Details Handling Images back to top
Product cited in:

Liu, Bunnell, Gurniak, Berg: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998

Heyeck, Wilcox, Bunnell, Berg: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997

Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993

Images

Product Details anti-ITK Antibody Target Details ITK Application Details Handling References for anti-ITK Antibody (ABIN967287) back to top
Supplier Images
Western Blotting (WB) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (AA 1-26) (ABIN967287) Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clon...
Western Blotting (WB) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (AA 1-26) (ABIN967287) Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were firs...
Western Blotting (WB) image for anti-ITK antibody (IL2-Inducible T-Cell Kinase) (AA 1-26) (ABIN967287) anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (Image 3)