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Human ITK ELISA Kit

Recommended ITK Product (supplied by: Log in to see )

Antigen
IL2-Inducible T-Cell Kinase (ITK) ELISA Kits
  • Emt
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • Tcsk
  • Tsk
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
4
3
Methode Type
Sandwich ELISA
Application
ELISA
Supplier
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Catalog No. ABIN4886151
$ 854.04
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012075712 ABIN4886152 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
0.0012075712 ABIN4886150 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
0.0012075712 ABIN1164599 Log in to see

Similar ITK ELISA Kits

Application / Reactivity Human
ELISA 4 ELISA Kits

General

Antigen IL2-Inducible T-Cell Kinase (ITK) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(4), (3)
Methode Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details ITK ELISA Kit

Target details Application Details Handling Images
Purpose Human Phosphotyrosine ITK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine ITK in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
ProductDetails: Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-ITK.
Characteristics
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Biotinylated Anti-Phosphotyrosine Antibody
  • Stop Solution
  • Assay Diluent(s)
  • Positive Control Sample
  • Lysis Buffer
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details ITK ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name ITK (ITK ELISA Kit Abstract)
Gene ID 3702
UniProt Q08881
Research Area Signaling, Tyrosine Kinases
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details ITK ELISA Kit Target details Handling Images back to top
Plate Strips (12 x 8)
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details ITK ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months